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TACT Genomics delivers innovative, high-quality biological products, solutions, and services for efficient, cost-effective biological research or new drug discovery.

Empower Biological Research

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Facilitate Molecular Bio-medicine

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Assist in Diagnosis

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NGS Library Preparation

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Product Highlights

Molecular Cloning Solution

DNA Preparation

Genomic DNA Isolation

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Plasmid DNA Isolation

#DC102 - FastPure Blood/Cell/Tissue/

Bacteria DNA Isolation Mini Kit

#DC104 - FastPure Plant DNA Isolation Mini Kit

#DC201 - FastPure Plasmid Mini Kit

RNA isolation

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#RC112 - FastPure Cell/Tissue Total RNA Isolation Kit V2, 6 min

cDNA synthesis

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R412 - HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper), 18 min

High Fidelity Phanta PCR Amplification

2 × Phanta UniFi Master Mix

     [#P516/P526(Dye Plus)]

DNA fragment clean up

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DC301 - FastPure Gel DNA Extraction Mini Kit and PCR product purification 

Seamless Cloning / Mutagenesis

#C117 - ClonExpress Ultra One Step Cloning Kit V3

Workflow of Seamless Cloning

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A. Vector Preparation, B. DNA Preparation, C. Recombination, D. Transformation

Colony PCR Screening & Genotyping PCR

#P222 - 2 × Rapid Taq Master Mix

Cell Biology > Cell Transfection

Transfection is the introduction of any nucleic acid molecule (DNA or RNA) into cultured eukaryotic cells utilizing means non- viral infection. 

TL301 - Lipomaster 3000 Transfection Reagent

Third-generation liposome transfection reagent for difficult-to-transfect cells

 Fig 1. TL301 - Lipomaster 3000 Transfection Reagent

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 Fig 2. Transfection results of Vazyme #TL301, Supplier A and Vazyme #TL201 in different cells

Product Description

Lipomaster 3000 Transfection Reagent is a liposome-mediated transfection reagent. It is applicable for plasmid DNA and RNA transfection of various adherent or suspension cells. With a clear structure, Lipomaster 3000 Reagent can achieve efficient and highly reproducible cell transfection. T3000 Enhancer Reagent, as a transfection reagent enhancer, can assist DNA to enter the nucleus quickly. The combination of two reagents can achieve higher transfection efficiency for large fragment plasmids and difficult-to-transfect cells. Lipomaster 3000 Transfection Reagent has the excellent ability to form liposome/nucleic acid complexes and rapid release of nucleic acid transfected into cells, which ensures excellent transfection performance and extremely low cytotoxicity. The presence of serum and antibiotics does not affect its transfection effect, so the formed liposome/nucleic acid complex can be directly added to the complete medium.

Product Features

#1. Superior performance: Efficient transfection of large plasmids and difficult-to-transfect cells #2. Broad compatibility: Only one reagent can be used for transfection of plasmid DNA, mRNA, siRNA, and co-transfection, etc. #3. Keep cell viability: Gentle on cells, low toxicity

Cell-based Luciferase Assay

DD1205 - Duo-Lite Luciferase Assay System

 Fig 1. Components of DD1205 - Duo-Lite Luciferase Assay System

Applications

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 Fig 2. Characterization of Transcription Factor by Measuring Transcriptional Activity

Detection Principle

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 Fig 3. Bioluminescent reactions catalyzed by firefly and Renilla luciferases

Product Description

Duo-Lite Luciferase Assay System is a highly sensitive, stable and uniform dual reporter gene detection kit (Fig 1&2). The kit first detects Firefly luciferase with luciferin as a substrate, then terminates the catalytic reaction of firefly luciferase, and then detects Renilla luciferase with coelenterazine as a substrate, thereby achieving the detection of dual luciferase reporter genes (Fig 2&3).  

Duo-Lite Luciferase Reagent is directly added to the cells in growth medium
to lyse the cells and provide firefly luciferase substrate, and the half-life of the generated optical signal can usually reach 2 hours.  fter that, Duo-Lite Stop & Lite Reagent is added to quench the luminescence of firefly luciferase reaction (the quench efficiency is more than 10,000 times), and the substrate of Renilla luciferase is provided, and the generated optical signal could also be read within 2 hours. Therefore, Duo-Lite Luciferase Assay System is more suitable for high-throughput
analysis of 96-or 384-well plates (Fig 4).

Product Features

  • Simple operation: Simply add the sample plate to complete the experiment without lysing the cells in advance.

  • Light signal stability: Glow kit has a fluorescence half-life of 2 hours, making it ideal for high-throughput operations.

  • High accuracy: The system contains renin fluorescein, which can correct errors caused by the number of cells between wells, transfection efficiency, cell growth status, etc.

Workflow

 Fig 4 Working flowchart when using DD1205 - Duo-Lite Luciferase Assay System

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