TACT Genomics delivers innovative, high-quality biological products, solutions, and services for efficient, cost-effective biological research or new drug discovery.
Empower Biological Research
Facilitate Molecular Bio-medicine
Assist in Diagnosis
NGS Library Preparation
Product Highlights
Molecular Cloning Solution
DNA Preparation
Genomic DNA Isolation
Plasmid DNA Isolation
#DC102 - FastPure Blood/Cell/Tissue/
Bacteria DNA Isolation Mini Kit
#DC104 - FastPure Plant DNA Isolation Mini Kit
#DC201 - FastPure Plasmid Mini Kit
RNA isolation
#RC112 - FastPure Cell/Tissue Total RNA Isolation Kit V2, 6 min
cDNA synthesis
R412 - HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper), 18 min
High Fidelity Phanta PCR Amplification
2 × Phanta UniFi Master Mix
[#P516/P526(Dye Plus)]
DNA fragment clean up
DC301 - FastPure Gel DNA Extraction Mini Kit and PCR product purification
Seamless Cloning / Mutagenesis
#C117 - ClonExpress Ultra One Step Cloning Kit V3
Workflow of Seamless Cloning
A. Vector Preparation, B. DNA Preparation, C. Recombination, D. Transformation
Colony PCR Screening & Genotyping PCR
#P222 - 2 × Rapid Taq Master Mix
Cell Biology > Cell Transfection
Transfection is the introduction of any nucleic acid molecule (DNA or RNA) into cultured eukaryotic cells utilizing means non- viral infection.
TL301 - Lipomaster 3000 Transfection Reagent
Third-generation liposome transfection reagent for difficult-to-transfect cells
Fig 1. TL301 - Lipomaster 3000 Transfection Reagent
Fig 2. Transfection results of Vazyme #TL301, Supplier A and Vazyme #TL201 in different cells
Product Description
Lipomaster 3000 Transfection Reagent is a liposome-mediated transfection reagent. It is applicable for plasmid DNA and RNA transfection of various adherent or suspension cells. With a clear structure, Lipomaster 3000 Reagent can achieve efficient and highly reproducible cell transfection. T3000 Enhancer Reagent, as a transfection reagent enhancer, can assist DNA to enter the nucleus quickly. The combination of two reagents can achieve higher transfection efficiency for large fragment plasmids and difficult-to-transfect cells. Lipomaster 3000 Transfection Reagent has the excellent ability to form liposome/nucleic acid complexes and rapid release of nucleic acid transfected into cells, which ensures excellent transfection performance and extremely low cytotoxicity. The presence of serum and antibiotics does not affect its transfection effect, so the formed liposome/nucleic acid complex can be directly added to the complete medium.
Product Features
#1. Superior performance: Efficient transfection of large plasmids and difficult-to-transfect cells #2. Broad compatibility: Only one reagent can be used for transfection of plasmid DNA, mRNA, siRNA, and co-transfection, etc. #3. Keep cell viability: Gentle on cells, low toxicity
Cell-based Luciferase Assay
DD1205 - Duo-Lite Luciferase Assay System
Fig 1. Components of DD1205 - Duo-Lite Luciferase Assay System
Applications
Fig 2. Characterization of Transcription Factor by Measuring Transcriptional Activity
Detection Principle
Fig 3. Bioluminescent reactions catalyzed by firefly and Renilla luciferases
Product Description
Duo-Lite Luciferase Assay System is a highly sensitive, stable and uniform dual reporter gene detection kit (Fig 1&2). The kit first detects Firefly luciferase with luciferin as a substrate, then terminates the catalytic reaction of firefly luciferase, and then detects Renilla luciferase with coelenterazine as a substrate, thereby achieving the detection of dual luciferase reporter genes (Fig 2&3).
Duo-Lite Luciferase Reagent is directly added to the cells in growth medium
to lyse the cells and provide firefly luciferase substrate, and the half-life of the generated optical signal can usually reach 2 hours. fter that, Duo-Lite Stop & Lite Reagent is added to quench the luminescence of firefly luciferase reaction (the quench efficiency is more than 10,000 times), and the substrate of Renilla luciferase is provided, and the generated optical signal could also be read within 2 hours. Therefore, Duo-Lite Luciferase Assay System is more suitable for high-throughput
analysis of 96-or 384-well plates (Fig 4).
Product Features
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Simple operation: Simply add the sample plate to complete the experiment without lysing the cells in advance.
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Light signal stability: Glow kit has a fluorescence half-life of 2 hours, making it ideal for high-throughput operations.
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High accuracy: The system contains renin fluorescein, which can correct errors caused by the number of cells between wells, transfection efficiency, cell growth status, etc.
Workflow
Fig 4 Working flowchart when using DD1205 - Duo-Lite Luciferase Assay System