FastPure EndoFree Plasmid Maxi Kit V2, SKU: DC212-C1
For large scale preparation of endotoxin-free and transfection-grade plasmid
Features
Transfection level : Extremely low endotoxin content (< 0.1 EU/μg DNA)
High yield: The yield of high copy plasmid is 0.2 mg-1.5 mg
Wide compatible: The input amount of bacterial cultures is 50 - 300 ml
Compared to DC202-01 Maxi prep kit, DC212--C1 has more features, such as
Easier operation: Invert and centrifuge
Lower centrifuge force: 8,000 x g
Product Manual
Plasmid DNA Maxi Kit - FastPure EndoFree Plasmid Maxi Kit V2, SKU: DC212
PRODUCT OVERVIEW
This kit is intended for extraction from 100 - 300 ml of overnight bacterial cultures. It adopts an optimized alkaline lysis method and features a unique silica membrane adsorption technology and special Buffer ERB and Buffer ERW for specific binding of plasmids and effective removal of endotoxins, proteins, and other impurities. The kit provides high yields of plasmid DNA with minimal endotoxin residue, suitable for use in transfection of various types of cells and common experiments such as enzyme digestion, PCR, sequencing, and ligation.
Storage
RNase A should be stored at -30 ~ -15°C and transported at ≤0°C.
Endotoxin Scavenger can be stored at 2 ~ 8°C for one month, stored at -30 ~ -15°C for long-term storage and transported at ≤0°C.
Other components should be stored at room temperature (15 ~ 25°C) and transported at room temperature.