Hyperactive ATAC-Seq Library Prep Kit for Illumina, SKU: TD711
This product is intended for studying chromatin accessibility / openness in mammalian cells with an input amount of 100 - 100,000 cells.
Product Overview
The Hyperactive ATAC-Seq Library Prep Kit for Illumina is a kit specifically developed for the Illumina high-throughput sequencing platform for studying chromatin accessibility/openness. ATAC-Seq, short for Assay for Transposase Accessible Chromatin with high-throughput sequencing, is a high-throughput sequencing technology that utilizes transposases to study chromatin accessibility. Its principle is based on the property of the transposase Tn5 to cleave open chromatin regions, allowing for the sequencing of DNA sequences from these open chromatin regions captured by the Tn5 enzyme. This kit optimizes the experimental reaction system and library preparation process, offering advantages over traditional techniques, including wider compatibility with cell input, shorter experimental cycles, higher signal-to-noise ratio, and broader application range. It is particularly suitable for epigenetic research in areas such as tumor diseases, biological development, and aging.
Components
See Table above.
Case Study
1. Starting Cell Input Number
ATAC libraries were constructed using HeLa cells at different input levels (100-100,000 cells) following the Vazyme #TD711 protocol. The amplification cycle number for different cell input levels is shown in Table 1. The results showed that TD711 library production was stable across different cell input levels, with the library peaks exhibiting a typical ladder-like distribution. Vazyme #TD711 has been optimized in both reagents and procedures, ensuring excellent library production and peak shape.
Figure 1. Library output under different cell input levels. Insert Table, Number of amplification cycles under different cell input levels
2. Improved sequencing data
The libraries obtained according to the Vazyme #TD711 experimental procedure were sequenced. After sequencing, 10 G of data was extracted from each sample for bioinformatics analysis. The results showed that: the TSS enrichment map showed significant enrichment around the transcription start sites of genes; the IGV view showed that the enrichment of ATAC at key sites was consistent with CUT&Tag and CUT&RUN, and the corresponding gene sites could be found in mRNA-seq, indicating that the experimental results were reliable.
Figure 2. TSS view under different cell input levels
Figure 3. IGV view under different cell input levels
Product User Manual
Hyperactive ATAC-Seq Library Prep Kit, SKU: TD711
Highlights
Applications
For rapid and efficient detection of open and easily accessible chromatin
- Open chromatin atlas
- Nucleosome localization
- Transcription factor footprint
Features
1. Easy to Use
• Optimized and simplified experimental protocols enable all researchers to easily perform ATAC-Seq detection
• Different experimental protocols optimized for cell or tissue samples
• No special or expensive equipment required
2. Rapid Results
• Competes preparation of sequencing libraries from samples in just 2.5 hours
3. Complete Kit
• Includes all reagents and enzymes
• The kit contains assembled transposons containing Tn5 transposase loaded with NGS adapters
4. Starting Cell Number
• Compatible with 100-100,000 cells.
5. Optimized Sequencing Data
• Good TSS enrichment and high signal-to-noise ratio in IGV views.
Storage
BOX 1：
Store 1% Digitonin at -30 ~ -15°C; it can be stored at room temperature (15 ~ 25°C) for one week.
Store the other components at -30 ~ -15°C and transport at ≤0°C.
BOX 2：
Store at 2 ~ 8°C and adjust the shipping method according to the destination.
