Lipomaster 3000 Transfection Reagent, SKU: TL301
Third-generation liposome transfection reagent for difficult-to-transfect cells
PRODUCT OVERVIEW
Lipomaster 3000 transfection reagent is a liposome-mediated transfection reagent suitable for transfecting plasmid DNA and RNA into various adherent or suspension cells. The Lipomaster 3000 transfection reagent has a well-defined structure, enabling highly efficient and reproducible cell transfection. The T3000 enhancer acts as a transfection enhancer, promoting rapid DNA entry into the cell nucleus. The combination of these two reagents significantly improves the transfection efficiency of large plasmid fragments and difficult-to-transfect cells.
Lipomaster 3000 Transfection Reagent has the excellent ability to form liposome/nucleic acid complexes and rapid release of nucleic acid transfected into cells, which ensures excellent transfection performance and extremely low cytotoxicity.
The presence of serum and antibiotics does not affect its transfection effect, so the formed liposome/nucleic acid complex can be directly added to the complete medium.
Components
See Table above.
Product Manual
Citation
98/100 Bioz Rating, 147 Citations by Sep 26, 2026. See the updated citation link.
Highlight
Direct activation of SARM1 by dsDNA is not supported by biochemical and cellular evidence.
Allison H Kao, Biswa P Mishra, ..., Aaron DiAntonio
Proceedings of the National Academy of Sci… , 2026 Sep 11, PMID 42726653, https://doi.org/10.1073/pnas.2620112123
Intracellular sclerostin promotes tumor progression and metastasis as a potential therapeutic target in triple-negative breast cancer.
Meiheng Sun, Hang Luo, ..., Ge Zhang
Cell Reports Medicine, 2026 Apr 23, DOI: 10.1016/j.xcrm.2026.102763
A non-coding variant at 2p24.2 confers susceptibility to non-syndromic cleft lip and palate through LLPS-dependent regulation of MYCN
Wu Zhaoyi, Yuan Zhiying, ..., He Miao
medRxiv, 2026 Apr 07, https://doi.org/10.64898/2026.04.07.26350283
Counteracting FOX proteins epigenetically control the herpesvirus lytic-latent balance
Yuhang Xiang, Xiyuan Yang, ..., Dongli Pan
Nature Communications, 2026 Feb 02. https://www.nature.com/articles/s41467-026-68915-1
Adipocytic sclerostin loop3-LRP4 interaction required by sclerostin to impair whole-body lipid and glucose metabolism.
Hewen Jiang, Xiaohui Tao, ..., Bao-Ting Zhang
Nature communications, 2026 Jan 16, https://www.nature.com/articles/s41467-026-68526-w
EGR Proteins Mediate Interferon-Independent Anti-HSV-1 Responses Through Viral and Host Targets.
Shuaishuai Wang, Fujun Hou, ..., Dongli Pan
Advanced Science, 2026 Jan 04, https://doi.org/10.1002/advs.202515546
Spatial control of m 6 A deposition on enhancer and promoter RNAs through co-acetylation of METTL3 and H3K27 on chromatin.
Xiang Huang, Jie Zhang, ..., Jinkai Wang
Molecular Cell, 2025 May 13, 85(7):1349-1365.e10. doi: 10.1016/j.molcel.2025.02.016
Lipomaster 3000 Transfection Reagent, SKU: TL301
Highlights
Features
Superior transfection efficiency: Works for large plasmids & difficult-to-transfect cells.
Broad compatibility: Only one reagent can be used for transfection of plasmid DNA, mRNA, siRNA, and co-transfection, etc, and be applicable for adherent or suspension cells with extremely low cytotoxicity.
Serum and antibiotics does not affect transfection effect: Formed liposome/nucleic acid complex can be directly added to the complete medium.
Keep cell viability: Gentle on cells, low toxicity
Applications
Cell types transfected with TL301: 293T, A549, BHT101, BRL-3a, BSR-T7, C2C12, COS7, DF1, H9C2(2-1), HaCaT, HCT116, Hela, Hepg2, HEK-293T, HL-02, HUH7,IPEC-J2, Jurkat, K562, MA-104, MC38, MC3T3-E1, MCF-7, MDA_MB_231, MDCK, NCI-H1299, NCI-H929, NIH3T3, NSCLC, PC-3, PC9/GR, PK15, RAW264.7, SNU387, U87mg, Vero
TL301-Lipid-based lentivirus transfection workflow diagram. See figure above.
Storage
Store a 2 ~ 8°C.
Adjust the shipping method according to the destination.
Note
Please invert this product upside down several times to mix thoroughly before each use. Do not freeze.
