One Step Mouse Genotyping Kit V2, SKU: PD111
Direct PCR kit compatible with multiple templates
PRODUCT OVERVIEW
The One-Step Mouse Genotyping Kit V2 is designed for rapid genotyping in mice. This kit contains lysis buffer for rapid release of genomic DNA from mouse tissues such as tail, ear, toe, and liver, and is also suitable for plant leaves, blood, and 293 cell samples. The lysis buffer can be used directly as a PCR amplification template after centrifugation. This kit contains 2× Rapid Taq Plus Master Mix (with dye), which includes next-generation hot-start Taq DNA polymerase and an optimized buffer system. Amplification can be performed simply by adding primers and template, reducing pipetting operations and improving throughput and reproducibility. Repeated tests have shown that this kit is widely applicable for amplifying complex genotypes and fragments up to 8 kb in size. Furthermore, this product contains tracer dyes for direct electrophoretic analysis after the reaction.
Components
See Table above.
Performance
See Table above.
Time-saving: Finish in ~1.5 h
Easy-to-Use: Add sample and run
High Performance: Sensitive, specific and reliable
Versatile: for mouse, plant and more
Applications
Mouse genotyping, transgenic detection and gene knockout analysis, etc.
Cell gene knockout identification
Plant transgenic identification
Product Manual
One Step Mouse Genotyping Kit V2, SKU: PD111
Highlights
Features
Equipped with 2 × Rapid Taq Plus Master Mix (Dye Plus): Contains a new generation hot-start Taq DNA polymerase and an optimized buffer system.
High efficiency: Rapid 3-minute lysis + 15 sec/kb amplification!
High success rate: Compatible with various tissue samples, blood, cells, and plant leaves; widely applicable for amplifying complex genotypes and fragments up to 8 kb in size.
Multiplex PCR: Supports 4- to 6-plex amplification.
Hot start: Dual-blocking antibody enables superior specificity and sensitivity.
FAQ & Troubleshooting
● No amplification products or low yield
The amount of sample to be lysed is relatively small: it is advisable to increase the input amount of lysis samples and the amount of template in the amplification system. Taking the mouse tail as an example, the recommended amount of lysis buffer in 50 μl can lyse 1 - 2 mm sample, 50 μl of lysis buffer can be compatible with 1.5 - 2 times the sample, and does not affect subsequent PCR amplification.
The impurities of lysate products are relatively high: try diluting the lysate products 2 - 10 times before performing PCR amplification.Annealing temperature set too high: appropriately decrease annealing temperature at 2°C intervally.
Poor primer design: use the purified mouse genome as a template to set a positive control and verify the primer effectiveness.
● Nonspecific products or smeared bands
Reaction system is prepared at room temperature: preparing the reaction system on ice can significantly reduce non-specific amplification.
Inappropriate annealing temperature setting: appropriately increase annealing temperature at 2°C intervally.
Serious PCR primer mismatch: redesign primer.
● Negative control showed amplification
Reaction system contamination: replace each component of the reaction system one by one.
Existence of aerosol pollution: Conduct thorough cleaning of the experimental environment, consumables, etc.
Storage
Store at -30 ~ -15°C and ship at ≤0°C.
▲ Mouse Direct Lysis Buffer A, and Mouse Direct Lysis Buffer B should be stored at 2 ~ 8°C for long-term storage.
