Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro, SKU: TD904
Cost-effective – The CUT&Tag kit, intended for studying protein-DNA interactions, is compatible with low cell starting quantities 10 - 100,000 input cells and qPCR.
 
Product Overview
The Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro is a kit specifically developed for the Illumina high-throughput sequencing platform to study protein-DNA interactions. CUT&Tag (Cleavage Under Target & Tagmentation) technology uses a Protein A/G fused transposase to precisely target the target protein under antibody guidance and fragment DNA near the target site. This kit optimizes the experimental reaction system and library preparation process, offering advantages over traditional ChIP-Seq such as lower cell input, shorter experimental procedures, higher signal-to-noise ratio, and better reproducibility. It is particularly suitable for research fields such as early embryonic development, stem cells, tumors, and epigenetics. 
 
The method involved in this product has been granted an invention patent, patent number ZL 2023 1 0474512.0.
 
Components
See Table above.
 
Performance
 
1. Compatibility with Low Cell Input
For low-input 293 cells (10, 25, 50, 100 cells), TD904 was used for H3K4me3 antibody CUT & Tag experiments, and library distribution was detected using an Agilent 2100 Bioanalyzer. Results showed that TD904 constructed similar ladder-shaped libraries for low cell inputs (10, 25, 50, 100 cells), indicating that TD904 is compatible with experiments using low cell inputs. Furthermore, due to the use of pA/G-Tnp, targeted cleavage is more precise and reproducible; the upgraded extraction magnetic beads effectively recover small fragments with more complete peak shapes.
 
Figure 1. Library fragment distribution with input of 10, 25, 50, and 100 cells, respectively
 
 
Even with low cell inputs, such as 10 cells, TD904 still achieved significant TSS enrichment.
 
Figure 2. TSS enrichment heatmap with low cell input of TD904
 
 
IGV view results show that corresponding results can be obtained even with low cell input.
 
Figure 3. Global IGV view with low cell input
 
Figure 4. IGV site view with low cell input
 
2. Advantage of small fragment enrichment
Using 293 cells as a sample, 100 cells were input, and H3K4me3 antibody was used to analyze the inserted fragment <120 bp. The results showed that the library with inserted fragment <120 bp could provide effective data, and TD904 could effectively recover small fragments and obtain corresponding small fragment information.
 
Figure 5. IGV view of inserted fragment <120 bp
 
3. IGV Comparison of Multi-omics results
Using 293 cells as a sample, 100,000 cells were input for multi-omics and multi-target site comparison. The results showed that the data obtained by TD904 is reliable and has a high signal-to-noise ratio.
 
Figure 6. IGV comparison of multi-omics results
 
Product Manual
Manual_TD904 V23.1