Routinely monitor mycoplasma contamination.
Mycolor One-Step Mycoplasma Detector, SKU: D201
Show the Mycoplasma some "colors"
PRODUCT OVERVIEW
Mycolor One-Step Mycoplasma Detector is a rapid detection product developed based on isothermal amplification technology for mycoplasma contamination in cell cultures. It has the advantages of easy operation, intuitive results, high sensitivity, strong specificity and anti-contamination. This product premixes enzymes with buffer to achieve one tube detection. After adding 1 μl of the cell culture supernatant to the reaction system and incubated at 65°C for 1 h, the results can be determined by visual observation. If there is mycoplasma contamination in the cell culture, the reaction solution will change from purple-red to yellow after amplification and the color contrast will be obvious. Meanwhile, the reagent introduces the anti-contamination system to avoid false positives caused by amplification product aerosols and ensure accurate and reliable results. Mycolor One-Step Mycoplasma Detector can detect various mycoplasma, including 8 strains commonly found in cell culture. This product has a wide range of adherent cell, suspension cell, media and serum compatibility.
Components
See Table above.
Workflow of Mycolor One-Step Mycoplasma Detector
See Video above.
Product Manual
Citation
95/100 Bioz Rating, 389 Citations by May 10, 2026. See the updated citation link.
Highlight:
1. Tissue-selective COPII modulator SEC16B aggravates cardiovascular disease by promoting lipid export.
Xiao Wang, Yating Hu, ..., Xiao-Wei Chen
The EMBO journal, 2026 Apr 24, https://doi.org/10.1038/s44318-026-00754-8
2. Mining of natural diversity enables efficient and expressible peptide asparaginyl ligases.Wenyu Du, Shi Qi, ..., Xinya HemuNature communications, 2026 Apr 27, https://doi.org/10.1038/s41467-026-72367-y3. Macrophage-mimetic photothermal nanotherapeutics regulate mitochondrial homeostasis and inflammatory cascades in lung ischemia-reperfusion injury.Haoxiang Yuan, Bo Zeng, ..., Jianxing HeCell Reports Medicine, 2026 Apr 23, DOI: 10.1016/j.xcrm.2026.1027684. Ammonia Detoxification Inhibits Liver Metastasis by Reshaping Hepatic Microenvironment.Sumin Sun, Haili Hu, ..., Liming Chen
Mycolor One-Step Mycoplasma Detector, SKU: D201
Highlights
Features
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Precise: Accuracy comparable to qPCR, better than PCR
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Easy to operate: One-tube; Compatible with multiple instruments
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Accuracy: With the anti-contamination system to effectively avoid false positives
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Easy to read the results: The contrast between negative (purple-red) and positive (yellow) is vivid.
Frequently Asked Questions
Detection principle
Q1: What is the detection principle of D201?
A1: The D201 is based on loop-mediated isothermal amplification (LAMP) technology. The reagent is premixed with Bst isothermal amplification enzyme, primers for amplification-detection of Mycoplasma (a pair of inner primers, a pair of outer primers, etc.) and optimized buffer. Just add the template, and then begin the LAMP reaction. When the mycoplasma DNA fragment is amplified, the color of the reagent in the reaction tube changes from purple-red to yellow. Therefore, the negative result for D201 is purple-red, and the positive result is yellow.
Sensitivity & Accuracy
Q2: What is the sensitivity of D201 (minimum detection limit)?
A2: As low as 100 copies/μl of Mycoplasma positive plasmid can be detected.
Q3: How is accuracy ensured?
A3: The product includes a negative and positive control, through which the result can be judged as accurate. We have tested 40 kinds of mycoplasma, including 8 kinds of common mycoplasma contamination.
Positive Control
Q4: Which mycoplasma sequence is the positive control in D201 based on?
A4: Positive control was plasmid containing M.hyorhinis sequence (concentration: 10 ng/μl)
Q5: Does the kit's positive control cause contamination?
A5: No, the positive control is mycogenic plasmid.
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Storage
Store at -30 ~ -15°C and transport at ≤0°C.
