Total RNA Extraction (Column based method) - FastPure Cell/Tissue Total RNA Isolation Kit V3,  SKU: RC122-01
Robust universal total RNA rapid purification kit (See Figure 1 above) 
 
PRODUCT OVERVIEW
The FastPure Cell/Tissue Total RNA Isolation Kit V3 utilizes silica membrane-based technology for rapid, high-quality purification of total RNA from animal tissues and cells, eliminating the need for hazardous reagents such as β-mercaptoethanol and phenol-chloroform.

Buffer RL (when used in conjunction with proteinase K) exhibits potent lysis activity, compatible with complex samples and high starting volumes. The FastPure column effectively removes contaminants and residual genomic DNA while achieving high RNA binding yields. Using optimized buffers, the resulting RNA is highly pure and free of residual contaminants, ready for direct use in RT-PCR, RT-qPCR, and microarray analysis.
 
Components
See Table above.
 
Product Manual
Manual_RC122 V26.1
 
Protocol of RNase-Free DNase Set for DNase treatment when using with Vazyme RNA purification kits
Protocol_RH104-C1_V26.1
 
Performance
 
Fast and Safe: Only Two Wash Steps Required
 
Total RNA can be extracted from various animal tissues and cells in just 10 minutes (the time indicated in the figure corresponds to lysis and centrifugation steps). No toxic reagents such as β-mercaptoethanol or phenol-chloroform are required, ensuring a safer laboratory workflow. See Figure 1 above.
 
High Extraction Success Rate
 
The Vazyme #RC122 kit reliably handles complex, difficult-to-lyse samples and is suitable for all common sample types. We used Vazyme #RC122 to extract RNA from a variety of animal tissue and cell samples. The purified RNA from various complex tissue and cell samples were analyzed by agarose gel electrophoresis. The results demonstrate that Vazyme #RC122 has broad compatibility with a wide range of animal tissue and cell samples. See Table 1 and Figure 2 above.
 
Reliable Performance for Downstream Applications: High Yield and Purity
 
Total RNA was isolated from various animal tissues and cell samples using Vazyme #RC122 and other commercially available total RNA extraction kits, following their respective operating procedures. The concentration and purity of the purified RNA were then assessed. The results, shown in Figure 3, indicate that Vazyme #RC122 yielded higher RNA extraction compared to similar products. Furthermore, the OD260/230 ratio of the RNA isolated using Vazyme #RC122 was closer to the ideal range, indicating higher purity.