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2 × Rapid Taq Plus Master Mix (Dye Plus), SKU: P223
2 x Rapid Taq Master Mix (Dye Plus), SKU: P223
Rapid Taq Plus DNA Polymerase is a new generation Taq DNA polymerase with a high amplification success rate.
 
PRODUCT OVERVIEW
 
Rapid Taq Plus DNA Polymerase is a new generation Taq DNA polymerase with a high amplification success rate. This product combines high blocking rate dual-species antibodies (binding different antigenic epitopes) and an optimized buffer system, demonstrating excellent success rate and specificity at an extension rate of 1 - 10 sec/kb. 
 
The pre-prepared 2 × Master Mix only requires the addition of primers and templates for PCR amplification, reducing pipetting operations and increasing detection throughput and result reproducibility. 
 
This product has robust amplification performance and high storage stability. It supports PCR amplification of genomic, plasmid, and λDNA templates up to 15 kb, and cDNA templates up to 10 kb. The unique protectant added to the product ensures that 2 × Master Mix does not freeze when stored at -20°C, allowing for convenient use. 
 
Additionally, this product contains tracking dyes, enabling direct electrophoresis analysis after the reaction. The amplification products are applicable for ClonExpress Ultra One Step Cloning Kit V3 (#C117) and Ultra-Universal TOPO Cloning Kit (#C603).
     
    Product Manual
    Manual_P223 V24.1

    2 × Rapid Taq Plus Master Mix (Dye Plus), SKU: P223

    SKU: P223-01
    C$183.00Price
    Quantity

    • Highlights

      Features

      • Rapid amplification: 1-10 sec/kb (<2 kb, 1 sec/kb; <5 kb, 5 sec/kb; ≤15 kb, 10 sec/kb);
      • Hot start: Dual-blocking antibody enables superior specificity and sensitivity;
      • Broad compatibility: Works with 24%-79% GC systems and 7-plex amplification;
      • Convenience: Store at -20°C without freezing, ready for immediate use and contains tracking dye. 

       

      Experimental Cases

      • Ultra-fast Amplification

        The 2 kb fragment was amplified using Vazyme #P223 (supplier A and supplier B) following the reaction procedures recommended in their respective manuals (Figure 1). P223 completes amplification in just 30 minutes, significantly reducing the required time.

       

      • Broad Compatibility

        The compatibility of low/high GC content DNA and different template types was tested using Vazyme #P223 and kits from suppliers A-E (Figure 2). The results showed that P223 was compatible with DNA templates with GC contents ranging from 24% to 79%, animal and plant lysates, and bacterial plaques/cultures. Its amplification success rate and specificity were superior to those of kits from suppliers A-E.

       

    • Storage

      Store at -30 ~ -15°C and transport at ≤0°C.

       

       

       

       

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