8 min FastPure Plasmid Mini Kit, DC221
8 min rapid plasmid DNA extraction for small scale preparation
Product Overview
This kit utilizes an optimized alkaline lysis method to extract high-purity plasmid DNA from 1-4 ml of overnight bacterial cultures, significantly improving nucleic acid release efficiency. A unique silica-based membrane adsorption technology effectively binds the plasmid while removing proteins, polysaccharides, and other impurities. A unique P2 indicator solution allows for visual confirmation of the completeness of lysis and neutralization, ensuring extraction quality and ease of use.
The extracted plasmid DNA yield is high, requiring only 8 minutes. The purified plasmid DNA can be directly used for restriction endonuclease digestion, PCR, sequencing, ligation, transformation, transfection of certain common cell lines, and other biological experiments.
Components
See Table 1 above.
Experimental Cases
- Rapid processing workflow: Extraction process completed within 8 minutes. See Figure 1.
- High yield of extracted plasmid DNA: See Figure 2.
- Easy to use:
Visual color confirmation indicates adequate lyse neutralization, and the compact flocculent precipitate enabling easier supernatant aspiration. See Figure 3.
Product User Manual
8 min FastPure Plasmid Mini Kit, DC221
Highlights
Features
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Rapid processing: Complete extraction in 8 min
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Excellent performance: Obtain up to 25 μg of high purity plasmid DNA
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Visualization: Easier supernatant aspiration with color indication
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Typical yields of different plasmids
Sample Input amount Yield pGBKT7 4 ml 19.0 μg pET28a 4 ml 4.5 μg pTT5 4 ml 16.2 μg pUC5-7 4 ml 14.5 μg Bds106 4 ml 11.5 μg 17K 4 ml 17.1 μg pCDNA3.1(+) 4 ml 25.8 μg pACT-eGFP 4 ml 19.1 μg L4440 4 ml 5.9 μg pLV[Exp]-Puro-
CMV>EGFP
4 ml 15.0 μg pGKJE8 4 ml 4.1 μg pBlueScriptII-SK(+) 4 ml 16.5 μg -
Storage
Store at 15 ~ 25°C and ship at room temperature.
