Plasmid DNA Mini Kit (column based method)
FastPure Plasmid Mini Kit, SKU: DC201-01
Cost-effective small scale preparation of plasmid
PRODUCT OVERVIEW
The FastPure Plasmid Mini Kit purifies 1 - 5 ml of cultured bacteria and lyses the cells by an optimized SDS-alkaline method. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, and binds plasmid DNA in a highly efficient manner under high-salt and low-pH conditions and largely remove proteins, genomic DNA, RNA, and other impurities. In the end, pure plasmid DNA is eluted from the silica membrane with a low salt, high-pH elution buffer.
The kit recovers up to 35 μg of high-copy plasmid DNA per isolation procedure. The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of common passage cells.
Product Manual
Citation
96/100 Bioz Rating, 283 Citations by May 10, 2026. Please see the updated citations.
Highlight
1. Maxillary palp odorant-binding proteins as key mediators of bitter taste and molecular targets for cockroach control.
Hong-Yan Feng, Ya-Qin Zhao, ..., Peng He
Journal of advanced research, 2026 Apr 27, https://doi.org/10.1016/j.jare.2026.04.063
2. Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation.
Wenya Zhang, Ling Yang, ..., Kai Lei
iScience, 2026 Apr 17, https://doi.org/10.1016/j.isci.2026.115274
3. Functional Characterization of VvSR34a Gene from Grapevine in Response to Salt Stress.
Yu Li, Zhen Gao, ..., Haibo Wang
Plants, 2026 Apr 02, DOI: 10.3390/plants15071092
4. EnvZ/OmpR-driven cooperative behavior promotes cefiderocol resistance in a hanging-droplet evolution system.
Bowen Li, Kaiwen Song, ..., Hui Wang
Science advances, 2026 Mar 27, DOI: 10.1126/sciadv.aea4527
Plasmid DNA Mini Kit - FastPure Plasmid Mini Kit, SKU: DC201
Highlights
Features
Fast and easy: No need for column equilibration; simple and fast extraction from multiple samples within 30 min;
High purity: Extracted plasmid DNA can be directly used in most molecular applications (sequencing, cloning, PCR);
High yield: up to 35 μg of plasmid DNA can be adsorbed per column.
Storage
Storage temperature is 15 ~ 25°C, shipped at room temperature.
If the ambient temperature often exceeds 25°C, we recommend storing RNase A at 2 ~ 8°C.
After adding RNase A, Buffer P1 is stable at 2 ~ 8°C for 6 months.
