Plasmid DNA Mini Kit (column based method)
FastPure Plasmid Mini Kit, SKU: DC201-01
Cost-effective small scale preparation of plasmid
PRODUCT OVERVIEW
The FastPure Plasmid Mini Kit purifies 1 - 5 ml of cultured bacteria and lyses the cells by an optimized SDS-alkaline method. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, and binds plasmid DNA in a highly efficient manner under high-salt and low-pH conditions and largely remove proteins, genomic DNA, RNA, and other impurities. In the end, pure plasmid DNA is eluted from the silica membrane with a low salt, high-pH elution buffer.
The kit recovers up to 35 μg of high-copy plasmid DNA per isolation procedure. The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of common passage cells.
Product Manual
Citation
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Plasmid DNA Mini Kit - FastPure Plasmid Mini Kit, SKU: DC201
Highlights
Features
- Fast and easy: No need for column equilibration; simple and fast extraction from multiple samples within 30 min;
- High purity: Extracted plasmid DNA can be directly used in most molecular applications (sequencing, cloning, PCR);
- High yield: up to 35 μg of plasmid DNA can be adsorbed per column.
Storage
Storage temperature is 15 ~ 25°C, shipped at room temperature.
If the ambient temperature often exceeds 25°C, we recommend storing RNase A at 2 ~ 8°C.
After adding RNase A, Buffer P1 is stable at 2 ~ 8°C for 6 months.
