Duo-Lite Luciferase Assay System, SKU: DD1205
Duo-Lite Luciferase Assay System: A glow dual luciferase reporter gene detection kit, featured by high sensitivity, stability and homogeneity, is easy to operate, and has a fluorescence half-life of 2 h, which is very suitable for high-throughput operation.
PRODUCT OVERVIEW
The Duo-Lite Luciferase Assay System is a kind of dual reporter gene detection kit featured by high sensitivity, stability and homogeneity. With high purity firefly luciferin and coelenterazine, firstly, the kit detects the firefly luciferase with luciferin as the substrate, and then terminates the catalytic reaction of firefly luciferase. At the same time, it can detect the renilla luciferase with coelenterazine as substrate, thereby realizing the double luciferase reporter gene detection.
Assay Workflow
See the Duo-Lite Luciferase Assay System's detection principle and workflow (Figure 1 & 2)
Product Manual
Summary
The Duo Lite Luciferase Assay System has emerged as a vital tool in scientific research, extensively employed to measure luciferase activity across diverse applications. This system has been instrumental in assessing gene expression, promoter activity, and regulatory mechanisms in fields such as immunology, oncology, virology, and plant biology. It has facilitated significant findings in understanding immune responses, signaling pathways, and gene interactions, contributing to insights into disease mechanisms, therapeutic potential, and novel treatment development.
The Duo Lite Luciferase Assay System has been crucial in quantifying viral replication, evaluating compound efficacy against viral infections, and assessing gene expression and regulation in various cellular contexts. Its applications span from determining the impact of microRNAs on gene expression to evaluating therapeutic antibodies and vaccines against viral infections. The system's reliability and accuracy have consistently supported the advancement of scientific knowledge, underscoring its importance in contemporary research.
Duo Luciferase Assay System, SKU: DD1205
Highlights
Features
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Simple operation: Just add sample and read the plate to complete the experiment, no need to lyse cells in advance.
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Light signal stability: The Glow kit has a fluorescence half-life of 2 hours, making it ideal for high-throughput operations..
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High accuracy: The system contains renin fluorescein, which can correct the errors caused by the number of cells between wells, transfection efficiency, cell growth status, etc.
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Storage
Storage temperature is -30~-15°C, transportation temperature ≤0°C.
Dissolving and dispensing reagents: long-term storage temperature is -85~-65°C, or short-term storage temperature is -30~-15°C (no more than one month).
Stop solution and reaction buffer: long-term storage temperature is 2~8°C.
