Duo-Lite Luciferase Assay System, SKU: DD1205
A glow dual luciferase reporter gene detection kit, featured by high sensitivity, stability and homogeneity, is easy to operate, and has a fluorescence half-life of 2 h, which is very suitable for high-throughput operation.
PRODUCT OVERVIEW
The Duo-Lite Luciferase Assay System is a kind of dual reporter gene detection kit featured by high sensitivity, stability and homogeneity. With high purity firefly luciferin and coelenterazine, firstly, the kit detects the firefly luciferase with luciferin as the substrate, and then terminates the catalytic reaction of firefly luciferase. At the same time, it can detect the renilla luciferase with coelenterazine as substrate, thereby realizing the double luciferase reporter gene detection.
Assay Workflow
See the Duo-Lite Luciferase Assay System's detection principle and workflow (Figure 1 & 2)
Product Manual
Summary
The Duo Lite Luciferase Assay System has emerged as a vital tool in scientific research, extensively employed to measure luciferase activity across diverse applications. This system has been instrumental in assessing gene expression, promoter activity, and regulatory mechanisms in fields such as immunology, oncology, virology, and plant biology. It has facilitated significant findings in understanding immune responses, signaling pathways, and gene interactions, contributing to insights into disease mechanisms, therapeutic potential, and novel treatment development.
Citation
96/100 Bioz Rating, 376 Citations by Nov 01, 2026. Please see the updated citations.
Highlights
TLR2 inhibition attenuates NET-driven inflammation and restenosis during poly-lactic acid bioresorbable scaffold degradation
Changyi Zhou, Rui Wang, ..., Junbo Ge
Bioactive Materials | 2026 Dec 01 | PMID 42569198 | Read Article
SAV1 in Cooperation With FKBP52 Participates in Implantation
Yuhan Shao, Yafang Lu, ..., Jia Yuan
Advanced Science | 2026 Sep 16 | Read Article
Programmable ZBTB7A RNA N 6 -methyladenosine site-specific demethylation suppresses colorectal cancer liver metastasis
Xuena Chen, Shuo Zhao, ..., Huili Hu
Molecular cancer | 2026 Aug 14 | PMID 42649501 | Read Article
Restoring Iron Homeostasis via Smoothened Inhibition: A Novel Strategy Against Hearing Loss
Huanyu Mao, Xiang Li, ..., Wenyan Li
Advanced Science | 2026 Apr 17 | PMID 41995135 | Read Article
Downregulation of miR-194-5p impairs cardiomyocyte proliferation and differentiation through targeting Fign in congenital heart disease.
Peishan Liang, Guangli Ren, ..., Xiangmeng Qu
Molecular and cellular biochemistry, 2026 Apr 04, https://doi.org/10.1007/s11010-026-05529-7
Sequential sequencing reveals the architecture and complexity of genomic variants in patients with Alport syndrome.
Hongling Di, Zhen You, ..., Zhihong Liu
Nature communications, 2026 Mar 23, https://www.nature.com/articles/s41467-026-70936-9
The CD97-PPM1G axis dampens antiviral immunity by dephosphorylating IRF7 in type I interferon pathway.
Huasong Chang, Wenjing Qi, ..., Hongbin He
PLOS Pathogens, 2026 Mar 03, https://doi.org/10.1371/journal.ppat.1014032
Targeting NAT10 alleviates colonic senescence and elderly-onset colitis by disrupting N4-acetylation of DYRK1A.
Jingyu Chen, Meng Xue, ..., Shujie Chen
Nature communications, 2026 Mar 01, https://www.nature.com/articles/s41467-026-70220-w
ZBTB21 Is a Dual Suppressor of Pyroptosis and MHC-I Antigen Presentation That Promotes Tumor Immune Evasion.
Lei Zhao, Linlin Sheng, ..., Hui Tian
Advanced Science, 2026 Feb 08, https://doi.org/10.1002/advs.202519836
Inhibition of STING-mediated antiviral innate immunity activation by CD97 via modulation of ER-phagy.
Huasong Chang, Rukun Yang, ..., Hongbin He
Communications biology, 2025 Dec 18, https://www.nature.com/articles/s42003-025-09380-2
A broadly neutralizing antibody confers cross-genus protection against alphaherpesviruses by inhibiting gB-mediated membrane fusion.
Guosong Wang, Yu Li, ..., Yixin Chen
Nature communications. 2025 Dec 16, https://www.nature.com/articles/s41467-025-66099-8
Engineering a streamlined virus-like particle for programmable tissue-specific gene delivery.
Jiabao Han, Hanyu Bai, ..., Wei Li
Nature communications, 2025 Oct 15, https://www.nature.com/articles/s41467-025-64181-9
Engineering of mRNA vaccine platform with reduced lipids and enhanced efficacy.
Xu Ma, Shaoli Liu, ..., Tianjiao Ji
Nature Communications, 2025 Oct 07, https://www.nature.com/articles/s41467-025-63965-3
Structure and function of a pair of non-competing monoclonal antibodies against Langya henipavirus attachment glycoprotein.
Wenting Li, Songyue Wu, ..., Zheng Zhang
Cell reports, 2025 Oct 09, https://doi.org/10.1016/j.celrep.2025.116407
Duo Luciferase Assay System, SKU: DD1205
Highlights
Features
Simple operation: Just add sample and read the plate to complete the experiment, no need to lyse cells in advance.
Light signal stability: The Glow kit has a fluorescence half-life of 2 hours, making it ideal for high-throughput operations..
High accuracy: The system contains renin fluorescein, which can correct the errors caused by the number of cells between wells, transfection efficiency, cell growth status, etc.
Storage
Storage temperature is -30~-15°C, transportation temperature ≤0°C.
Dissolving and dispensing reagents: long-term storage temperature is -85~-65°C, or short-term storage temperature is -30~-15°C (no more than one month).
Stop solution and reaction buffer: long-term storage temperature is 2~8°C.
