Duo-Lite Luciferase Assay System, SKU: DD1205
A glow dual luciferase reporter gene detection kit, featured by high sensitivity, stability and homogeneity, is easy to operate, and has a fluorescence half-life of 2 h, which is very suitable for high-throughput operation.
 
PRODUCT OVERVIEW
The Duo-Lite Luciferase Assay System is a kind of dual reporter gene detection kit featured by high sensitivity, stability and homogeneity. With high purity firefly luciferin and coelenterazine, firstly, the kit detects the firefly luciferase with luciferin as the substrate, and then terminates the catalytic reaction of firefly luciferase. At the same time, it can detect the renilla luciferase with coelenterazine as substrate, thereby realizing the double luciferase reporter gene detection.
 
Assay Workflow
See the Duo-Lite Luciferase Assay System's detection principle and workflow (Figure 1 & 2)

Product Manual
Manual_DD1205_V24.1
 
Summary
The Duo Lite Luciferase Assay System has emerged as a vital tool in scientific research, extensively employed to measure luciferase activity across diverse applications. This system has been instrumental in assessing gene expression, promoter activity, and regulatory mechanisms in fields such as immunology, oncology, virology, and plant biology. It has facilitated significant findings in understanding immune responses, signaling pathways, and gene interactions, contributing to insights into disease mechanisms, therapeutic potential, and novel treatment development. 
 
Citation
96/100 Bioz Rating, 343  Citations by May 12, 2026. Please see the updated citations.
Highlight
1. Downregulation of miR-194-5p impairs cardiomyocyte proliferation and differentiation through targeting Fign in congenital heart disease.
Peishan Liang, Guangli Ren, ..., Xiangmeng Qu
Molecular and cellular biochemistry, 2026 Apr 04, https://doi.org/10.1007/s11010-026-05529-7 
2. Sequential sequencing reveals the architecture and complexity of genomic variants in patients with Alport syndrome.
Hongling Di, Zhen You, ..., Zhihong Liu
Nature communications, 2026 Mar 23, https://www.nature.com/articles/s41467-026-70936-9 
3. The CD97-PPM1G axis dampens antiviral immunity by dephosphorylating IRF7 in type I interferon pathway.
Huasong Chang, Wenjing Qi, ..., Hongbin He
PLOS Pathogens, 2026 Mar 03, https://doi.org/10.1371/journal.ppat.1014032 
4. Targeting NAT10 alleviates colonic senescence and elderly-onset colitis by disrupting N4-acetylation of DYRK1A.
Jingyu Chen, Meng Xue, ..., Shujie Chen
Nature communications, 2026 Mar 01, https://www.nature.com/articles/s41467-026-70220-w
5. ZBTB21 Is a Dual Suppressor of Pyroptosis and MHC-I Antigen Presentation That Promotes Tumor Immune Evasion.
Lei Zhao, Linlin Sheng, ..., Hui Tian
Advanced Science, 2026 Feb 08, https://doi.org/10.1002/advs.202519836 
6. Inhibition of STING-mediated antiviral innate immunity activation by CD97 via modulation of ER-phagy.
Huasong Chang, Rukun Yang, ..., Hongbin He
Communications biology, 2025 Dec 18, https://www.nature.com/articles/s42003-025-09380-2
7. A broadly neutralizing antibody confers cross-genus protection against alphaherpesviruses by inhibiting gB-mediated membrane fusion.
Guosong Wang, Yu Li, ..., Yixin Chen
Nature communications.  2025 Dec 16, https://www.nature.com/articles/s41467-025-66099-8
8. Engineering a streamlined virus-like particle for programmable tissue-specific gene delivery.
Jiabao Han, Hanyu Bai, ..., Wei Li
Nature communications, 2025 Oct 15, https://www.nature.com/articles/s41467-025-64181-9
9. Engineering of mRNA vaccine platform with reduced lipids and enhanced efficacy.
Xu Ma, Shaoli Liu, ..., Tianjiao Ji
Nature Communications, 2025 Oct 07, https://www.nature.com/articles/s41467-025-63965-3
10. Structure and function of a pair of non-competing monoclonal antibodies against Langya henipavirus attachment glycoprotein.
Wenting Li, Songyue Wu, ..., Zheng Zhang
Cell reports, 2025 Oct 09, https://doi.org/10.1016/j.celrep.2025.116407