Mut Express Universal Fast Mutagenesis Kit, SKU: C216
The ideal choice for site-directed mutagenesis.
Product Overview
The Mut Express Universal Fast Mutagenesis Kit, based on ClonExpress homologous recombination technology, allows for simultaneous site-directed mutagenesis of 1-5 discontinuous sites on a target plasmid in a single operation. This kit integrates the amplification module of 2×Phanta Flash Master Mix and the rapid cloning module of 2×CE Mix V3. 2×Phanta Flash Master Mix offers high fidelity and rapid amplification, and is a single-tube mixture requiring only the addition of primers and template. When using the Mut Express Universal Fast Mutagenesis Kit for site-directed mutagenesis, primer design is flexible, and the input template amount is extremely low, facilitating the complete degradation of the original methylated template. 2×CE Mix V3 can accommodate 1-5 site-directed mutagenesis. If the amplification products are specific, the Dpn I digestion products can be used directly for recombination reactions without DNA purification, making it the preferred kit for multi-site DNA mutagenesis.
Components
See Table above.
Primer Design Software
Primer Design for Single or Multiple Point Mutations
Product Manual
Citation
Highlight
1. SLC13A2-transported citrate remodels transcriptional regulation through protein acetylation to suppress tumor growth.
Mengyao Qin, Longcheng Shang, ..., Jing Xiong
Science Advances, 2026 Apr 10. DOI: 10.1126/sciadv.aec4368
2. LSM14B is an Oocyte-Specific RNA-Binding Protein Indispensable for Maternal mRNA Metabolism and Oocyte Development in Mice
Hui Li, Hailian Zhao, ... and You-Qiang Su
Advanced Sciences. 2023 Jun;10(18):e2300043. doi: 10.1002/advs.202300043.
3. Wogonin derivative V8 enhances bortezomib efficacy in gastric carcinoma by disrupting lysosome-mediated drug resistance.
Si-Chan Li, Shun-Zi Shao, ..., Xiao-Bo Zhang
World Journal of Gastroenterology, 2026 Feb 10. DOI: 10.3748/wjg.v32.i8.113299
4. Structure and function of a pair of non-competing monoclonal antibodies against Langya henipavirus attachment glycoprotein.
Wenting Li, Songyue Wu, ..., Zheng Zhang
Cell reports, 2025 Oct 09, https://doi.org/10.1016/j.celrep.2025.116407
Mut Express Universal Fast Mutagenesis Kit, SKU: C216
Highlights
Features
Universal system: Compatible with 1-5 mutation sites;
High efficiency: A single-site mutation on a 10 kb plasmid can be completed in just 1.5 h;
Simple operation: Amplified product can be directly used in the recombination reaction after being digested with DpnI;
Widely applicable: Suitable for amplification of any site in the plasmid within 20 kb.
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
