Total RNA Extraction (Column based method) - FastPure Cell/Tissue Total RNA Isolation Kit V2, SKU: RC112-01
Easily extract high-quality RNA from cells and animal tissues in 6 min (fast and non-toxic) (See Figure 1)
Features
- Fast: It only requires 6 min at room temperature;
- Safer: No need for β - mercaptoethanol and phenol or chloroform;
- Minimal gDNA residue: No need for on-membrane digestion with DNase I, one-step gDNA contamination removal;
- High RNA purity: High purity of extracted RNA, which is ready for use in demanding downstream experiments;
- Widely applicable: Compatible with all kinds of cell and tissue samples
Components
See Table above.
Performance
- Super Fast
Obtain one samples in just 6 min at room temperature (See Figure 2)
- High Quality
Vazyme #RC112 ('V' in Figure 3) and commercially available related products (from suppliers A, B, C, D, E, F, and G respectively) were used to extract total RNA from the rat liver tissue (10 mg) and HEK 293 cell (1×106) samples according to the respective Instructions for Use. Figure 3A is the agarose gel electrophoretogram of the extracted RNA, while Figures 3B and 3C are the statistical charts of RNA concentration and purity results. As shown in the figures, RNA extracted by Vazyme #RC112 has good integrity, high yield and high purity.
- Wide Sample Compatibility
Vazyme #RC112 was used for the RNA extraction and purification of different samples, and the final RNA product was detected by agarose gel electrophoresis (Figure 4). It shows that Vazyme #RC112 is well compatible in the extraction of RNA from different cell and animal tissue samples.
Product Manual
Total RNA extraction - FastPure Cell/Tissue Total RNA Isolation Kit, SKU: RC112
PRODUCT OVERVIEW
The FastPure Cell/Tissue Total RNA Isolation Kit V2 provides a rapid method for extracting total RNA from human and animal tissues, and cells. The kit is based on silica gel membrane purification technology and does not require b-mercaptoethanol, phenol/chloroform, or any other toxic reagent during the extraction process. It takes only 6 min to extract high-quality RNA. The kit contains FastPure gDNA-Filter Columns III which can effectively remove impurities and gDNA. FastPure RNA Columns III can efficiently bind RNA with an optimized buffer to obtain high-purity total RNA. The isolated RNA has little gDNA residues and no proteins or other impurities contamination. It can be used for RT-PCR, Real-Time PCR, Microarrarys and other downstream experiments.
Storage
Store at 15 ~ 25°C and transport at room temperature.