Total RNA Extraction (Column based method) - FastPure Cell/Tissue Total RNA Isolation Kit V2, SKU: RC112-01
Easily extract high-quality RNA from cells and animal tissues in 6 min (fast and non-toxic) (See Figure 1)
PRODUCT OVERVIEW
The FastPure Cell/Tissue Total RNA Isolation Kit V2 provides a rapid method for extracting total RNA from human and animal tissues, and cells. The kit is based on silica gel membrane purification technology and does not require b-mercaptoethanol, phenol/chloroform, or any other toxic reagent during the extraction process. It takes only 6 min to extract high-quality RNA. The kit contains FastPure gDNA-Filter Columns III which can effectively remove impurities and gDNA. FastPure RNA Columns III can efficiently bind RNA with an optimized buffer to obtain high-purity total RNA. The isolated RNA has little gDNA residues and no proteins or other impurities contamination. It can be used for RT-PCR, Real-Time PCR, Microarrarys and other downstream experiments.
Components
See Table above.
Product Manual
Protocol of RNase-Free DNase Set for DNase treatment when using with Vazyme RNA purification kits
Citation
99/100 Bioz Rating. 3,062 Citations by May 5, 2026. See the updated citation link.
Highlight:
1. An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice.
Xiaojuan Wang, Guohui Zhang, ..., Henry M Krause, Jiabao Liu
Nature Communications. 2025 Feb 3;16:1280. doi: 10.1038/s41467-025-56624-0
2. Macrophage-mimetic photothermal nanotherapeutics regulate mitochondrial homeostasis and inflammatory cascades in lung ischemia-reperfusion injury.
Haoxiang Yuan, Bo Zeng, ..., Jianxing He
Cell Reports Medicine. 2026 Apr 23, https://pubmed.ncbi.nlm.nih.gov/42030937/
3. MTPN drives noncanonical ERK hyperactivation in colorectal cancer and provides a promising therapeutic approach for precision medicine in CRC.
Chushu Li, Shouyan Deng, ..., Fenglin Chen
Oncogene, 2026 Apr 20, https://www.nature.com/articles/s41388-026-03795-9
4. Multiomics profiling and experiments in preclinical models revealed RAD51-IN-1 as a synergistic potentiator of anlotinib sensitivity.
Huangyang Meng, Qianjing Chang, ..., Wenjun Cheng
Science Advances, 2026 Apr 17, https://www.science.org/doi/10.1126/sciadv.aeb0855
Frequently Asked Questions (FAQS)
A: 200 μg
A: It can remove DNA with an input of 15 μg, with an efficiency exceeding 99.5%.
Q: How do I remove DNA using DNase I?
A: TACT Genomics/Vazyme provides a DNase I kit, which can be used according to the RH104-C1 protocol.
Q: How to improve RNA yield?
A: ● For samples with high fibrous content, such as muscle tissue, we recommend the following extraction steps to obtain higher yields: Take approximately 10 mg of tissue, add 300 μl of buffer RL, vortex to mix, then add 590 μl of RNase-free ddH2O and 10 μl of proteinase K (#DE102), mix well, and incubate at 56°C for 10-20 minutes. For subsequent steps, please refer to the manual, 08-2/RNA Extraction section.
● For insect samples, please refer to the manual, 08-2/RNA Extraction/Step 2. For liver samples, please use 1 volume of 50% ethanol instead of 0.5 volume of 100% ethanol.
● For bacterial samples, we recommend using a bacterial RNA enhancement reagent (#R412-C1) to obtain better recovery rates. The specific procedure is as follows: Centrifuge to collect 1-1.5 ml of bacterial culture precipitate. Add 200 μl of preheated (95°C) auxiliary reagent to the precipitate to resuspend the bacteria. Incubate at 95°C for 4 minutes (not recommended to exceed 5 minutes). Then add 300 μl of Buffer RL and mix thoroughly. Follow the instructions in the manual, section 08-2/RNA extraction.
● For samples with high RNase content, such as pancreas, spleen, and small intestine, it is recommended to add 1% β-mercaptoethanol to Buffer RL before proceeding with the subsequent steps in the manual.
FastPure Cell/Tissue Total RNA Isolation Kit, SKU: RC112
Highlights
Features
- Fast: RNA extraction only takes 6 min at room temperature;
- Safer: No need for β - mercaptoethanol and phenol or chloroform;
- Minimal gDNA residue: No need for on-membrane digestion with DNase I, one-step gDNA contamination removal;
- High RNA purity: High purity of extracted RNA, which is ready for use in demanding downstream experiments;
- Widely applicable: Compatible with all kinds of cell and tissue samples
Performance
- Super Fast
Complete RNA extraction from one sample in just 6 min at room temperature (See Figure 2)
- High Quality
Vazyme #RC112 ('V' in Figure 3) and commercially available related products (from suppliers A, B, C, D, E, F, and G respectively) were used to extract total RNA from the rat liver tissue (10 mg) and HEK 293 cell (1×10^6) samples according to the respective Instructions for Use. Figure 3A is the agarose gel electrophoretogram of the extracted RNA, while Figures 3B and 3C are the statistical charts of RNA concentration and purity results. As shown in the figures, RNA extracted by Vazyme #RC112 has good integrity, high yield and high purity.
- Wide Sample Compatibility
Vazyme #RC112 was used for the RNA extraction and purification of different samples, and the final RNA product was detected by agarose gel electrophoresis (Figure 4). It shows that Vazyme #RC112 is well compatible in the extraction of RNA from different cell and animal tissue samples.
Storage
Store at 15 ~ 25°C and transport at room temperature.
