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Phanta Ultra Master Mix, SKU: P518
High-fidelity PCR
 
2 × Phanta Ultra Master Mix , SKU: P518
Precision and Speed Perfectly Balanced (~200x Taq fidelity)
 
Product Overview
2× Phanta Ultra Master Mix is ​​a next-generation high-fidelity DNA polymerase carefully selected based on the BioSmart platform, offering ultra-high fidelity, rapid amplification at 5 seconds/kb, and broad Tm compatibility. Combining the latest hot-start technology and a superior buffer system, this product exhibits high success rates and specificity at universal annealing temperatures, amplifying fragments up to 40 kb in length. Its fidelity is 200 times higher than Taq polymerase, providing an efficient and convenient solution for PCR experiments requiring high-precision amplification, such as molecular cloning, sequencing, and site-directed mutagenesis. The amplified products are blunt-ended and can be directly used in the ClonExpress Ultra One Step Cloning Kit V3 (Vazyme #C117) and Ultra-Universal TOPO Cloning Kit (#C603).
 
Components 
See the Table above.
 
Purchase Guide
High Fidelity Enzyme Product Selection Guide. See the Guide Figure 4.
 
Product Manual
Manual_P518 V25.1
 

Phanta Ultra Master Mix, SKU: P518

SKU: P518-01
CA$141.00Price
Quantity

  • Highlights

    Features  

    • Ultra-high Fidelity: ~200x higher than Taq DNA Polymerase;

    • Ultra-fast Amplification: <10 kb, 5 sec/kb;  ≥10 kb, 10 sec/kb;

    • Universal Program: Universal primer annealing at 60°C;

    • Broad Compatibility: Suitable for complex samples, GC-rich/poor systems, and multiplex amplification

     

    Performance

    • Ultra-High Fidelity

      The fidelity amplification of different polymerases was determined using the next-generation sequencing method (P. Mielinis. et al. Journal of Molecular Biology (2021)). The result shows that the fidelity of Phanta Ultra DNA Polymerase is ~200 times that of Taq DNA Polymerase. See Figure 1 above.

     

    • Ultra-fast Amplification

      For ~15 kb fragments, Phanta Ultra can save up to 7 hours of PCR time!  See Figure 2 above.

     

    • Universal Program

      2 × Phanta Ultra Master Mix (Vazyme #P518)/2 × Phanta Ultra Master Mix (Dye Plus) (Vazyme #P528) were used for parallel comparison with Supplier A, Supplier B. The results show that P518 and P528 have broad Tm com-patibility and outperform Suppliers A, and B. They can efficiently amplify multiple fragments of varying lengths and Tm values on a single PCR machine. See Figure 3 above.

     

  • Storage

    Store at -30 ~ -15°C and transport at ≤0°C.

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