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Rapid PCR - 2 x Rapid Taq Master Mix, SKU: P222
2 x Rapid Taq Master Mix, SKU: P222
2 x Rapid Taq Master Mix uses an enhanced DNA polymerase with an extension time of 15 seconds/kb, and even faster extension times up to 1 second/kb for fragments ≤1 kb.
 
Product Overview
The 2 x Rapid Taq Master Mix contains Taq DNA Polymerase, elongation promoting factor, dNTP, and optimized buffer system. The amplification speed of this product can reach 15 seconds/kb, which is suitable for rapid PCR. The amplification speed can reach up to 1 second/kb for DNA fragments within 1kb, greatly saving reaction time.
 
The 2 x Rapid PCR Master Mix only needs to add primers and templates for amplification, which reduces pipetting operations and improves detection throughput and result reproducibility.
 
This kit has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5kb with genome as template and PCR amplification within 10kb with plasmid and λDNA as template. The protective agent added to the system enables 2 x Master Mix to maintain stable activity after repeated freezing and thawing.
 
This product contains loading buffer and tracking dye. After the reaction, the PCR product can be directly loaded for electrophoresis, which is convenient to use.
 
The PCR product has adenine at the 3' end and can be cloned into the T vector. It is compatible with ClonExpress (#C116/C117/) and TOPO cloning kits (#C603).
 
Compared to 2 x Rapid Taq Master Mix, the Plus version delivers enhanced performance and greater convenience. If you are having trouble genotyping a particular sample or amplifying a specific complex DNA fragment, consider using P223 - 2× Rapid Taq Plus Master Mix (Dye Plus).
 
Components
See above Table.
 
Product Manual
Manual_P222 V22.1
 
Citation
98/100 Bioz Rating, 728 Citations  by May 10, 2026.  See the updated citation link. 
 
Highlight:
1. Intracellular sclerostin promotes tumor progression and metastasis as a potential therapeutic target in triple-negative breast cancer.
Meiheng Sun, Hang Luo, ..., Ge Zhang
Cell Reports Medicine, 2026 Apr 23,  DOI: 10.1016/j.xcrm.2026.102763  
2. Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccination
Hong Kai Teo, Shuting Han, ..., Gloryn Chia
Science Advances, 2026 Apr 17,  Vol 12, Issue 16. DOI: 10.1126/sciadv.adz1156
3.  A Non-Canonical Role of SMAD4 in Regulating 3D Genome Architecture to Inhibit Lung Squamous Cell Carcinoma Development
Tang Qian, Lian Chen, ..., Liu Jian
bioRxiv, 2026 Mar 31,   https://doi.org/10.64898/2026.03.28.714793
4.  Neuron-Specific WWOX Gene Therapy Produces Dose-Dependent, Durable Rescue in a Model of WWOX-Related Epileptic Encephalopathy
Obeid Mustafa, Akkawi Rania, ..., Aqeilan Rami I.
bioRxiv, 2026 Mar 13,  https://doi.org/10.64898/2026.03.11.710995 
5.  Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens
Moran Anfang, Reem Haj Yahya, ..., Eilon Shani
Cell Reports, 2026 Mar 09,  45(3):117055. doi: 10.1016/j.celrep.2026.117055
6. A light-responsive transcriptional circuit suppresses sexual reproduction in the rice blast fungus in the field.
Hao Liang, Penghui Zhang, ..., Shi-Hong Zhang
Cell Reports, 2026 Feb 27, https://doi.org/10.1016/j.celrep.2026.117038

Rapid PCR - 2 x Rapid Taq Master Mix, SKU: P222

SKU: P222-01
CA$151.00Price
Quantity

  • Highlights

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

    Features

    • Fast amplification: 15 sec/kb; extension time up to 1 sec/kb (≤ 1kb fragment);

     

    • Convenience: Includes tracking dye, PCR products are loaded directly onto gel;

     

    • Good stability: Stable performance after 50 freeze-thaw cycles;

     

    • Ready-to-use: 2 × Master Mix format simplifies workflow and minimizes pipetting steps.

     

     

     

  • Storage

     

    Store at -30 ~ -15°C and transport at ≤0°C.

     

     

     

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