VAMNE MagUltra Circulating Cell-free DNA Isolation Kit, SKU: N913
Flexible, High-Yield, High-Throughput, Capturing Circulating Cell-free DNA (See Figure 1)
 
Features:
Flexible input volume: Compatible with 0.2 - 10 ml of serum and plasma, 2 - 10 ml of urine, and other cell-free liquid samples.
Efficient recovery of target fragments: High yield and excellent recovery of small fragments, with consistent and reliable quality for downstream analysis.
Non-toxic: Free from toxic organic solvents such as phenol and chloroform, and does not require the addition of isopropanol.
Automated and efficient: Compatible with 24-well nucleic acid extraction instrument, enabling the processing of up to 24 samples in just 1 hour.
 
Components
See Table above.
 
Performance
Flexible input volume
 
The Vazyme #N913-VAMNE MagUltra circulating free DNA isolation kit was used in conjunction with the market-leading Vendor A to extract 0.2, 0.5, 1, 2, 4, and 10 ml plasma samples. Extracted nucleic acid yield was quantified using the Qubit assay, and fragment distribution was analyzed using an Agilent 2100 Bioanalyzer (Figures 2A and 2B). The results demonstrate that Vazyme #N913 achieved comparable yield and quality to Vendor A at all tested input amounts.
 
Efficient recovery of target fragments
To evaluate the performance of Vazyme #N913, simulated samples were prepared by spiking dsDNA fragments of different sizes (80 bp, 170 bp, and large gDNA fragments) into 2 ml PBS, with a final target fragment concentration of 10 ng/ml. A 2 ml plasma sample was processed in parallel for comparison. Vazyme #N913 was compared with commercially available products from Supplier A, D, F, and G in terms of target fragment purification. The concentration of the purified products was measured using Qubit (Figure 3 & 4A ), and the fragment distribution was analyzed with Agilent 2100 Bioanalyzer (Figure 4B). The results showed that Vazyme #N913 exhibited superior recovery of small fragments compared to Suppliers D, F, and G, and performed comparably to Supplier A. Additionally, it showed significantly lower recovery of non-target large gDNA fragments compared to the other products. The fragment analysis confirmed that the target DNA purified with Vazyme #N913 was equivalent or superior than other suppliers, with undetectable large gDNA contamination.
 
Superior downstream data quality
Vazyme #N913 was compared with market-leading products (Supplier A, D, F, G) in the extraction from 2 ml plasma samples (including both normal and hemolyzed samples). After quantification with Qubit, Vazyme #ND627 library preparation kit and Vazyme #N321 adapter kit were performed. Captured libraries underwent sequencing, and then data were analyzed. The results showed that N913 performed comparably to Supplier A, D, F, and G in library yield, GC content, and Q30 values. In terms of duplication rate, N913 showed similar performance to Supplier A for normal samples, and outperformed Suppliers D, F, and G (Figure 5A). For hemolyzed samples, N913 demonstrated superior performance over all compared suppliers (Figure 5B).  
 
Figure Legends
Figure 1. N913 features and product image.
Figure 2. Comparison of extraction efficiency and peak profiles between N913 and similar products at different plasma sample input volumes. Note: The elution volume of the 10 ml input system is twice that of the 4 ml system.
Figure 3. Comparison of DNA fragment recovery rates between N913 and similar products at different DNA fragment sizes.
Figure 4. Comparison of DNA extraction yield and peak profiles between N913 and similar products using plasma samples.
Figure 5. Comparison of downstream sequencing quality between N913 and competing products. Note: A: Library preparation data for normal samples (library yield, duplicate reads rate, GC content, and Q30 value); B: Library preparation data for hemolyzed samples (library yield, duplicate reads rate, GC content, and Q30 value). 
 
Product Manual
Manual_N913_V25.1