High-Throughput Automated DNA and RNA Extraction

VAMNE Magnetic EndoFree Plasmid Mini Kit, SKU: DC201-C

Semi-automated plasmid extraction from 1 - 5 ml of overnight bacterial cultures.

Product Overview

VAMNE Magnetic EndoFree Plasmid Mini Kit is designed for the extraction of plasmid DNA from 1 - 5 ml of overnight bacterial cultures. It employs an optimized alkaline lysis method to efficiently lyse cells, followed by purification using uniquely embedded superparamagnetic, silica-based magnetic beads that specifically bind plasmid DNA while effectively removing proteins and other impurities. Only 5 min of manual operation is required, and the nucleic acids can be obtained within 45 min when combined with the subsequent automated program, making the process simple and efficient. The uniquely formulated Buffer AP2 contains an indicator dye that enables visual monitoring of the lysis and neutralization steps through color changes, ensuring optimal extraction quality and visualized operation. The purified plasmid DNA is suitable for enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of conventional passaging cells.

Components

See the component Tables above for DC201-C1 and DC201-C2, respectively.

• DC201-C1 for VNP-96P

• DC201-C2 for VNP-32P

Performance

High yield, integrity, and purity

The actual plasmid yield, integrity and purity of DC201-C2 are equal to or better than those of most competitive products. See Figure 1 above.

Broad Compatibility

DC201-C2 is compatible with extracting different plasmid samples and yields plasmids that are equal to or higher than competitive products. See Figure 2 above.

Downstream Applications

The transfection efficiency of plasmids extracted using DC201-C2 is comparable to that of competitive products. See Figure 3 above.

Storage

Store at 15 ~ 25 °C and ship at room temperature.

Product Manual

Manual_DC201-C1 V26.1

Manual_DC201-C2 V26.1

Program_DC201-C1 V26.1

Program_DC201-C2 V26.1