UniPeak U+ One Step RT-qPCR SYBR Green Kit, SKU: Q226
Ultra-Sensitive, Contamination-Resistant One-Step Dye RT-qPCR Master Mix for Unmatched Specificity.
PRODUCT OVERVIEW
The UniPeak U+ One-Step RT-qPCR SYBR Green Kit is a dye-based RT-qPCR reagent designed for quantitative PCR detection using RNA as a template. Dye-based quantitative PCR faces two major challenges: difficulty in detecting low-concentration samples and non-specific amplification. This product utilizes a next-generation antibody-modified Taq DNA polymerase and a one-step temperature-activated reverse transcriptase, combined with an optimized RT-qPCR buffer system, effectively addressing these challenges and significantly improving detection sensitivity and specificity. To further ensure the accuracy of quantitative results, this product also includes a dUTP/UDG anti-contamination system to eliminate aerosol contamination at room temperature. Additionally, a 5× gDNA Wiper effectively removes residual genomic DNA contamination from the RNA template. The 2× One-Step SYBR Green Master Mix contains tracer dyes and a universal ROX reference dye, making it compatible with all types of qPCR instruments; simply add primers and template for amplification.
Components
See the table above.
Performance
- Ultra-Sensitive, Highly Specific One-Step RT-qPCR
We conducted comparative tests on Q226 and supplier A's product, covering three systems with different GC contents and one non-specific system. See Figure 1. The results showed that Q226 exhibited superior sensitivity compared to its competitor. Furthermore, in the non-specific system, its melting curve displayed a single peak, indicating high specificity.
- Equipped with UDG anti-contamination system
When testing 4, 40, and 400 pg of all-U and all-T templates, the contamination prevention efficiency was calculated using the formula: E = (1 - 2^(-ΔCT(all-U - all-T)))%. Q226 achieved a contamination prevention efficiency exceeding 99% at all concentrations. See Figure 2.
- Incorporating a gDNA removal module for more reliable results
No amplification curve was observed after processing 100 ng of gDNA template with the gDNA removal module, indicating that the gDNA Wiper in Q226 achieved 100% removal efficiency. See Figure 3.
Products manual
UniPeak U+ One Step RT-qPCR SYBR Green Kit, SKU: Q226
Highlights
Features
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Superior Performance: Designed for high sensitivity and specificity, ensuring reliable amplification across a wider range of systems.
- Anti-Contamination: Equipped with an advanced dUTP/UDG system to effectively prevent amplification product contamination.
- Genomic DNA Removal: Includes a genomic DNA removal module to remove residual genomic DNA, ensuring accurate results.
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Storage
Store at -30 ~ -15°C and ship at ≤0°C.
2 × One Step SYBR Green Master Mix store at -30 ~ -15°C, protect from light.
