TUNEL BrightRed Apoptosis Detection Kit, SKU: A113
Brighter & stable red fluorescent TUNEL apoptosis detection
Features
• Bright factor: Brighter fluorescence, stronger anti-fade ability
• Red fluorescent label: Easy co-labeling with green fluorescents such as GFP
• Efficient: Highly active recombinant TdT enzyme ensures fluorescence incorporation efficiency
• Broad compatible: Suitable for cell slides, paraffin sections and frozen sections
TUNEL BrightRed Apoptosis Detection Kit, SKU: A113
PRODUCT OVERVIEW
During apoptosis, intracellular specific endonucleases are activated, chromatin DNA is specifically cleaved between nucleosomes, and DNA is degraded into integer multiples of about 180 - 200 bp fragments. The 3'-hydroxyl (3'-OH) end generated by broken DNA can bind to tetramethylrhodamine-deoxyuridine triphosphate (TMR red-dUTP) under the action of terminal deoxynucleotidyl transferase (TdT). Broken DNA labeled with TMR red-dUTP can be directly observed by fluorescence microscopy or quantitatively analyzed by flow cytometry to reflect the level of apoptosis.
The BrightRed Labeling Mix contains TMR red-dUTP and Bright factor. This unique small molecule compound can non-covalently bind to TMR red-dUTP, enhance its stability and amplify its signal, resulting in brighter labeled fluorescence and stronger anti-quenching ability.
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
BrightRed Labeling Mix: Store at -30 ~ -15°C and protect from light.