PCR-Related
SwiftCut DpnI, SKU: C404
High-Fidelity restriction enzymes (digestion in 5-15 minutes)
PRODUCT OVERVIEW
SwiftCut series is a type of fast restriction endonuclease that is obtained by recombination-mediated genetic engineering. All SwiftCut endonucleases can accurately cleave DNA within 5 - 15 min, which are applicable for fast restriction endonuclease digestion of plasmid DNA, PCR products, and genomic DNA. All SwiftCut series endonucleases have 100% activity in the universal 10 × SwiftCut Buffer or 10 ×SwiftCut Color Buffer, allowing multiple restriction endonuclease digestions to be performed simultaneously in the same reaction system. In addition, 10 × SwiftCut Color Buffer contains red and yellow tracking dyes, facilitating direct gel electrophoresis of PCR products. The red dye in the 10 × SwiftCut Color Buffer migrates at a similar rate to 2,500 bp double-stranded DNA fragments in a 1% agarose gel, and the yellow dye migrates at a similar rate to 10 bp double-stranded DNA fragments in a 1% agarose gel.
Components
See the Table above.
Product Manual
SwiftCut DpnI, SKU: C404
Highlights
Features
Fast: Digestion time as short as 5 – 15 minutes, saving experimental time.
Convenient: Universal buffer supports all restriction endonucleases, simplifying multi-enzyme reaction.
SwiftCut color buffer: Include tracking dye for direct loading of digestion products on gels.
Applications
The restriction endonuclease digestion of genomic DNA and plasmid DNA.
The removal of plasmid templates after amplification reaction.
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
