Protein A/G Magnetic Beads, PB101
Suitable for IP/CoIP/ChIP, high specificity and low background!
 
Features
High binding capacity: Good binding effects, clear bands, minimal antibody loss (binding efficiency ≥0.5mg/ml) 
 
Good specificity: Single target strip, without non-specific bands, low background
 
Performance
Low Background Interference
Using Vazyme #PB101 Protein A/G Magnetic Beads, phospho-Akt was immunoprecipitated from 1 mg of 293T cell lysate. After high-temperature denaturation and elution in 1× SDS-PAGE Sample Loading Buffer, an anti-light chain secondary antibody was used to analyze phospho-Akt enrichment by Western blotting. The results showed that PB101 exhibited low nonspecific protein adsorption and no protein A/G dropout, resulting in a cleaner background (Figure 1).
 
Excellent ChIP Signal-to-Noise Ratio
ChIP experiments  targeting H3K4me3 in 293T cells was performed using Vazyme #PB101 Protein A/G Magnetic Beads. The results demonstrated that PB101 is compatible with ChIP-seq experiments and exhibits an excellent signal-to-noise ratio (Figure 2 & 3).
 
High Binding Efficiency
Flag-tagged proteins were immunoprecipitated using Vazyme #PB101 Protein A/G magnetic beads in combination with Vazyme #RA1003-DYKDDDDK Tag Mouse mAb using 1 mg of 293T cell lysate protein. After high-temperature denaturation and elution in 1× SDS-PAGE Sample Loading Buffer, enrichment of Flag-tagged proteins was assessed by Western blotting using an anti-light chain secondary antibody. The results showed that PB101 proteins exhibited higher binding efficiency (Figure 4).
 
Product Manual
Manual_PB101 V24.1