miRNA Reverse Transcription
miRNA 1st Strand cDNA Synthesis Kit (by stem-loop), SKU: MR101
miRNA reverse transcription by stem-loop method
Product Overview
The miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a kit specifically designed for the synthesis of stem-loop miRNA cDNA single-strands. This kit includes a genomic DNA removal step, which rapidly removes genomic DNA contamination within 2 minutes at 42°C, ensuring the reliability of subsequent experimental results. The HiScript II reverse transcriptase used in this kit exhibits extremely high thermostability, and the optimized buffer system facilitates the generation of miRNA-specific reverse transcription products. For optimal experimental results, it is recommended to use the miRNA universal SYBR qPCR premix (#MQ101) for subsequent cDNA product quantification.
Components
See components above.
Online Primer Design Software
- Access the online primer design software via 'miRNA Primer Design'.
Product Manual
miRNA 1st Strand cDNA Synthesis Kit (by stem-loop), SKU: MR101
Highlights
Features
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Excellent linear relationship: It has a good linear relationship in a wide range of template input and can detect pg-level RNA templates.
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Superior reaction system: The optimized Buffer composition and concentration are more suitable for the reverse transcription of microRNA.
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Software support: Provide online primer design software 'miRNA Primer Design'.
Online Primer Design Software
- Access the online primer design software via 'miRNA Primer Design'.
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Storage
Store at 2 ~ 8°C and protect from light. Ship on ice pack.

