Reverse Transcription - HiScript IV RT SuperMix for qPCR (+gDNA wiper), SKU: R423
R423 - HiScriptIV Supermix for qPCR (+gDNA wiper) uses a simpler and more straightforward workflow with only two steps or pipettings to wipe gDNA and synthesize cDNA in 18 min.
It improves the cDNA synthesis efficiency, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates.
Features
- Superior reverse transcription efficiency: Achieve qPCR detection results with smaller CT values;
- Great gDNA elimination efficiency: Thoroughly remove 500 ng of gDNA to ensure the accuracy of qPCR detection;
- Excellent template compatibility: Efficient reverse transcription of low-input, low-expression or degraded RNA templates.
Components
See Table 1 above.
Performance
- Faster Reaction: HiScriptIV Supermix for qPCR (+gDNA wiper) uses a simpler and more straightforward workflow with only two steps or pipettings to wipe gDNA and synthesize cDNA in 18 min (Figure 1).
Superior RT Efficiency
100 ng RNA from animals, plants and microbes were reverse transcribed by Vazyme #R423 and similar RT products from Supplier A, Supplier B and Supplier C, following their respective manuals. The RT efficiency of these reagents was evaluated by qPCR amplification of obtained cDNA. It can be seen from the Figure 2, △CT between Vazyme #R423 and Supplier C is within 0.5, indicating a similar efficiency of them; △CT between Vazyme #R423 and Suppler A/Supplier B are more than 0.5, indicating a better efficiency of Vazyme #R423.
Great gDNA Elimination Efficiency
500 ng gDNA from 293T cells were eliminated by Vazyme #R423 and similar RT products from Supplier A, Supplier B and Supplier C. The digested gDNA were amplified by qPCR to evaluate the elimination efficiency of these products. The results show that the gDNA elimination efficiency of Vazyme #R423 is consistent with that of Supplier A and Supplier B, and significantly better than that of Supplier C (Table 2).
Excellent Template Compatibility
100 ng human FFPE samples and degraded 293T cells (RIN=1) were reverse transcribed by Vazyme #R423 and similar RT products from Supplier A, Supplier B and Supplier C. The template compatibility of these reagents was evaluated by qPCR amplification of obtained cDNA. The results show that △CT between Vazyme #R423 and Suppliers are more than 0.5 in most genes, indicating a better template compatibility of Vazyme #R423 (Figure 3).
Product Manual
HiScript IV RT SuperMix for qPCR (+gDNA wiper), SKU: R423
PRODUCT OVERVIEW
HiScript IV RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse Transcriptase and Buffer optimized for reverse transcription. This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5 x gDNA wiper Mix can completely remove the genomic contaimination in the RNA template, so there is no need to design intron-spanning qPCR primers. 4 x HiScript IV qRT SuperMix contains all the components required for the reverse transcription, just add template RNA and RNase-free ddH2O to start the reaction.
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
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