HiScript IV All-in-One Ultra RT SuperMix for qPCR, SKU: R433
Accelerate and Streamline, All-in-One: Convenience Meets High Performance.
Features
Faster Results: With an all-in-one reagent, complete genomic DNA removal and reverse transcription in just 5 minutes, accelerating your research progress.
Higher Efficiency: Produces higher yields of cDNA, leading to lower CT values and enabling accurate analysis of low-expression targets.
Components
See Table above.
Performance
Faster Reaction
Complete genomic DNA removal and reverse transcription in 5 minutes (Figure 1).
Total RNA from 293T cells (100 ng) was used as a template for reverse transcription reactions with Vazyme #R433, following the manufacturer’ s protocol at different reverse transcription times (5 min, 10 min, and 15 min) (Figure 2). The resulting cDNA was then subjected to qPCR quantification for multiple genes using Vazyme #Q712. The results demonstrate that Vazyme #R433 achieved effective reverse transcription at both the 5-minute rapid time and the 15-minute standard time.
Higher Reverse Transcription Efficiency
Reverse transcription reactions were conducted using 100 ng of RNA from animal cells, plant tissues, and animal tissues as templates with Vazyme #R433, Vazyme #R333, and other commercially available reverse transcription reagents (Supplier A and B) (Figure 3), following the recommended protocols for each product. The resulting cDNA underwent quantitative analysis of multiple genes through qPCR. The results indicate that Vazyme #R433 consistently yielded lower CT values across different testing systems, indicating superior reverse transcription efficiency compared to both Vazyme #R333 and the products from Supplier A and B.
Product Manual
Citation
Highlight
2. CHAMP1 complex promotes heterochromatin assembly and reduces replication stress.
Feng Li, Amira Elbakry, ..., Alan D. D’Andrea
Proceedings of the National Academy of Sci… January 2, 2026123 (1) e2525144122 https://doi.org/10.1073/pnas.2525144122
3. Loss-of-function mutations in PLD4 lead to systemic lupus erythematosus.
Qintao Wang, Honghao Zhu, ..., Zhihong Liu
Nature, 647, pages498–505, 2025 Sep 10, https://doi.org/10.1038/s41586-025-09513-x
HiScript IV All-in-One Ultra RT SuperMix for qPCR, SKU: R433
PRODUCT OVERVIEW
HiScript IV All-in-One Ultra RT SuperMix for qPCR is a next-generation reverse transcription kit specifically designed for qPCR. It integrates all the components required for genomic DNA removal and RNA reverse transcription in a single tube; simply add template and water to initiate the reaction. Leveraging a specially optimized reverse transcriptase, DNase, and proprietary buffer, it rapidly completes genomic DNA removal and reverse transcription in just 5 minutes. The resulting cDNA is compatible with both dye-based and probe-based qPCR methods.
Storage
Store at -30 ~ -15°C and ship at ≤0°C .
