HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper), SKU: R312
High efficiency universal RT-PCR/RT-qPCR mix with gDNA elimination module
Product Overview
The HiScript III First-Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of the HiScript II First-Strand cDNA Synthesis Kit (+gDNA wiper). It includes next-generation HiScript III reverse transcriptase and buffers optimized for reverse transcription, further improving first-strand synthesis efficiency.
The kit's 5× gDNA wiper Mix rapidly removes genomic DNA contamination within 2 minutes, resulting in more reliable results and simplifying qPCR primer design by eliminating the need to cross exon-exon junctions. The kit contains single-component reverse transcription primers Oligo(dT)20VN and random hexamers, allowing users to flexibly choose primers according to their experimental needs.
This kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly homogeneous cDNA for qPCR.
Components
See Table above.
Product Manual
HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper), SKU: R312
Highlights
Features
- Exceptional reverse transcription efficiency: Compatible with low RNA input and degraded RNA templates.
- Superior reverse transcription performance: Yields cDNA lengths up to 20 kb.
- High impurity tolerance: Maintains high reverse transcription performance even in the presence of common impurities such as ethanol, isopropanol, and water-balanced phenols.
- Optional primers: Oligo (dT)20VN and random hexamer primers are individually packaged.
- Highly efficient removal of genomic DNA: Effectively removes up to 500 ng of genomic DNA.
Storage
Store at -30 ~ -15°C and ship at ≤0°C .
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