HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper), SKU: R312
High efficiency universal RT-PCR/RT-qPCR mix with gDNA elimination module
Product Overview
The HiScript III First-Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of the HiScript II First-Strand cDNA Synthesis Kit (+gDNA wiper). It includes next-generation HiScript III reverse transcriptase and buffers optimized for reverse transcription, further improving first-strand synthesis efficiency.
The kit's 5× gDNA wiper Mix rapidly removes genomic DNA contamination within 2 minutes, resulting in more reliable results and simplifying qPCR primer design by eliminating the need to cross exon-exon junctions. The kit contains single-component reverse transcription primers Oligo(dT)20VN and random hexamers, allowing users to flexibly choose primers according to their experimental needs.
This kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly homogeneous cDNA for qPCR.
Components
See Table above.
Product Manual
Citation
Highlight
1. A rice ceRNA module suppresses Rhizoctonia solani-induced cross-kingdom RNAi to reduce fungal pathogenicity.
Jile Ni, Wanpeng Mao, ..., Qiang Cai
Nature communications, 2026 Apr 22, https://www.nature.com/articles/s41467-026-72158-5
2. Astrocytic AEBP1-NPAS3-LIPA pathway coordinates cholesterol homeostasis to regulate Alzheimer’s pathology
Jialin Wu, Xiaonan Lu, ..., Jian Sima
Cell Reports, 2026 Mar 24;45(4):117193. doi: 10.1016/j.celrep.2026.117193
3. Engineering a streamlined virus-like particle for programmable tissue-specific gene delivery.
4. Loss-of-function mutations in PLD4 lead to systemic lupus erythematosus.
Qintao Wang, Honghao Zhu, ..., Zhihong Liu
Nature, 647, pages498–505, 2025 Sep 10, https://doi.org/10.1038/s41586-025-09513-x
HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper), SKU: R312
Highlights
Features
- Exceptional reverse transcription efficiency: Compatible with low RNA input and degraded RNA templates.
- Superior reverse transcription performance: Yields cDNA lengths up to 20 kb.
- High impurity tolerance: Maintains high reverse transcription performance even in the presence of common impurities such as ethanol, isopropanol, and water-balanced phenols.
- Optional primers: Oligo (dT)20VN and random hexamer primers are individually packaged.
- Highly efficient removal of genomic DNA: Effectively removes up to 500 ng of genomic DNA.
Storage
Store at -30 ~ -15°C and ship at ≤0°C .
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