Total RNA Isolation - FreeZol Reagent, SKU: R711
Extract RNA at RT without using chloroform
Features
- Safe and low-toxic: No toxic or hazardous reagents such as chloroform and β-mercaptoethanol are required.
- Easy to use: Single-phase RNA extraction system eliminates the need for phase separation, and all procedures are performed at room temperature.
- High RNA quality: High RNA purity and integrity, with yields comparable to those of the traditional TRIzol method.
Performance
Easy and Non-toxic Workflow Figure 1
Superior Quality
The RNA of rat kidney, spleen, HepG2 and SP2/0 cells were extracted using Vazyme #R711 and other similar products (Supplier A, Supplier B, and Vazyme #R701) (Figure 2), respectively. And the concentration and integrity of RNA products were analyzed by agarose gel electrophoresis . The results show that the yield and integrity of RNA extracted by Vazyme #R711 are excellent.
Ultra lysis capability
129 sample types including cell, tissue, plant,and bacteria, etc. were validated.
Cultured Cells (Figure 3)
Animal Tissues (Figure 4)
Plants (Figure 5)
Product Manual
Total RNA Isolation - FreeZol Reagent, SKU: R711
Product Overview
FreeZol reagent is widely used to extract total RNA from cultured cells, animal tissues, and simple plant tissues. Compared to the traditional Trizol method, this product is simple to use, can be performed at room temperature, and does not require chloroform for phase separation. Furthermore, this product ensures the integrity and purity of the extracted RNA by precipitating impurities such as proteins, DNA, and polysaccharides in the organic phase, while retaining the RNA in the upper aqueous phase. The entire extraction process can be completed in less than 1 hour. The extracted total RNA can be directly used in molecular biology experiments such as RT-PCR, qRT-PCR, Northern blotting, Dot blotting, in vitro translation, and next-generation sequencing.
Storage
Store at 2~8°C and transport them at room temperature.