Total RNA Isolation - FreeZol Reagent, SKU: R711
Extract RNA at room temperature without using chloroform
Features
- Safe and low-toxic: No toxic or hazardous reagents such as chloroform and β-mercaptoethanol are required.
- Easy to use: Single-phase RNA extraction system eliminates the need for phase separation, and all procedures are performed at room temperature.
- High RNA quality: High RNA purity and integrity, with yields comparable to those of the traditional TRIzol method.
Performance
Easy and Non-toxic Workflow Figure 1
Superior Quality
The RNA of rat kidney, spleen, HepG2 and SP2/0 cells were extracted using Vazyme #R711 and other similar products (Supplier A, Supplier B, and Vazyme #R701) (Figure 2), respectively. And the concentration and integrity of RNA products were analyzed by agarose gel electrophoresis . The results show that the yield and integrity of RNA extracted by Vazyme #R711 are excellent.
Ultra lysis capability
129 sample types including cell, tissue, plant,and bacteria, etc. were validated.
Cultured Cells (Figure 3)
Animal Tissues (Figure 4)
Plants (Figure 5)
Product Manual
Citation
99/100 Bioz Rating, 2,815 Citations by May 10, 2026. See the updated citation link.
Highlight:
1. Extracellular Vesicles Enable CircRNA Delivery via in situ Biogenesis and Sorting
Li Minchao, Pan Yuxuan, ..., Li Zhenhua
bioRxiv. 2026 Apr 28, https://www.biorxiv.org/content/10.64898/2026.04.24.720573v1
2. METTL1-mediated m 7 G modification of ASNS mRNA regulates asparagine metabolism reprogramming to promote hepatocellular carcinoma progression.
Huiwu Xing, Fuqiang Ma, ..., Xinyu Gu
Oncogene. 2026 Apr 27, https://www.nature.com/articles/s41388-026-03806-9
3. A microbiota-IPA axis facilitates intestinal stem cell-mediated regeneration in colitis through a Hopx-associated program.
Yanan Zhang, Jinxin Meng, ..., Shu Jeffrey Zhu
Nature communications, 2026 Feb 25, https://www.nature.com/articles/s41467-026-70062-6
Total RNA Isolation - FreeZol Reagent, SKU: R711
Product Overview
FreeZol reagent is widely used to extract total RNA from cultured cells, animal tissues, and simple plant tissues. Compared to the traditional Trizol method, this product is simple to use, can be performed at room temperature, and does not require chloroform for phase separation. Furthermore, this product ensures the integrity and purity of the extracted RNA by precipitating impurities such as proteins, DNA, and polysaccharides in the organic phase, while retaining the RNA in the upper aqueous phase. The entire extraction process can be completed in less than 1 hour. The extracted total RNA can be directly used in molecular biology experiments such as RT-PCR, qRT-PCR, Northern blotting, Dot blotting, in vitro translation, and next-generation sequencing.
Storage
Store at 2~8°C and transport them at room temperature.
