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FlysisAmp Cells-to-CT 2-Step SYBR Green Kit, SKU: CL122
FlysisAmp Cells-to-CT 2-Step SYBR Green Kit, SKU: CL122
Obtain cellular RNA in 7 min and CT in 65 min.
 
PRODUCT OVERVIEW
The FlysisAmp Cells-to-CT 2-Step SYBR Green Kit is a complete kit for rapidly and efficiently converting cells into amplification results, suitable for cultured cells of 10-10^6. Genomic DNA removal and RNA extraction can be performed directly in the cell culture plate. This kit utilizes a high-performance HiScript IV RT SuperMix for first-strand cDNA synthesis and amplification using a dye-based qPCR mixture, providing cellular gene expression results in as little as 67 minutes.

This kit simplifies the cumbersome steps of traditional extraction methods, improving efficiency and reducing sample loss during purification. It is suitable for various cell types (adherent or suspension cells) and cell numbers, and addresses the issue of unsatisfactory extraction results with low cell counts using traditional methods. This kit can be used for high-throughput (96-well/384-well cell culture plates) cellular RNA expression analysis.
 
Performance
  • Easy to operate: No need for traditional extraction steps such as tube transfer and centrifugation, the operation can be performed directly in the culture plate. The cell gDNA is cleared and RNA is lysed in 7-9 minutes. The lysate is directly used for 2-step RT-qPCR experiment. The whole process is as fast as 65 minutes.
 
  • Wide sample compatibility & high specificity: Paired with a universal dye-based RT-qPCR 2-step premix, the special additive ingredients significantly inhibit the formation of primer dimers, resulting in more accurate quantitative results and suitable for all cell types. 
 
Applications
It is applicable for gene expression analysis from 10 - 10^6 cultured cells (adherent or suspension cells).
 
Product manual
Manual_CL122 V24.1

FlysisAmp Cells-to-CT 2-Step SYBR Green Kit, SKU: CL122

SKU: CL122-01
C$1,196.00Price
Quantity

  • Highlights

     

     

     

     

     

     

    Features

    • Fast and simple operation: 67 min to finish from RNA to CT.

     

    • Wide sample compatibility: Compatible with 10 - 10^6 adherent/suspension cells.

     

    • High throughput: Compatible with different cell culture plates (384/96/24/12/6), RNA acquisition can be done directly in well plates.

     

    • High sensitivity: Combined with upgraded HiScript IV RT SuperMix and the universal dye-based qPCR premix for stable detection of low-expression systems, avoiding missed detections.

     

     

     

  • Storage

     

     

    Store at -30 ~ -15°C and transport at ≤0°C.

     

     

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