FlysisAmp Cells-to-CT 1-Step SYBR Green Kit, SKU: CL132
Easily obtain cellular RNA in 7 min and obtain qPCR results in as fast as 37 min (See Figure 1 & 2)
Features
- Convenient Operation: Obtain RNA quantification results from cells in as fast as 52 min.
- Wide Sample Compatibility: Compatible with 10 to 106 adherent and suspension cells.
- High Throughput: Compatible with various cell culture plates (384/96/24/12/6 wells), RNA can be obtained directly in the well plate.
- High Specificity: Combined with a dedicated premix for universal dye-based RT-qPCR one-step, it can inhibit primer dimers to a certain extent, resulting in more accurate quantitative results.
Performance
Easy to operate: No need for traditional extraction steps such as tube transfer and centrifugation, the operation can be performed directly in the culture plate. Only two steps are required, and the cell gDNA is cleared and RNA is lysed in 7-9 minutes. The lysate is directly used for one-step RT-qPCR experiment. The whole process is as fast as 52 minutes. See Figure 3.
Wide sample compatibility & high specificity: Paired with a universal dye-based RT-qPCR one-step premix, the special additive ingredients significantly inhibit the formation of primer dimers, resulting in more accurate quantitative results and suitable for all cell types. See Figure 4.
Applications
It is applicable for gene expression analysis from 10 - 106 cultured cells (adherent or suspension
cells).
Product manual
FlysisAmp Cells-to-CT 1-Step SYBR Green Kit, SKU: CL132
PRODUCT OVERVIEW
The FlysisAmp Cells-to-CT 1-Step SYBR Green Kit is a comprehensive kit that quickly and efficiently goes from "cells" to "amplification results," compatible with 10 to 106 cultured cells. Genomic DNA removal and RNA extraction can be performed directly in the cell culture plate. Combined with a highly efficient, stable, and specific dye-based RT-qPCR kit, gene expression results can be obtained from cells in as fast as 52 minutes.
This kit eliminates the tedious steps of traditional extraction, improving efficiency and reducing sample loss during purification. Compatible with a variety of cell types (adherent or suspension cells) and cell numbers, the kit addresses the issue of suboptimal extraction results due to low cell numbers in traditional extraction methods. This kit can be used for high-throughput (96-well/384-well cell culture plate) cellular RNA expression analysis.
Storage
Store at -30 ~ -15°C and transport at ≤0°C.