mRNA QC Reagents:
EasyAna T7 RNA Polymerase Quantitative Detection Kit (ELISA) SKU: DD3504EN
T7 RNA Polymerase (T7 RNA polymerase) is a DNA-dependent 5'→3' RNA polymerase that recognizes the T7 promoter sequence with high specificity ,can be used for transcriptional synthesis of mRNA in IVT.
Product Overview
T7 RNA Polymerase (T7 RNA polymerase) is a protein encoded by phage T7 DNA as expressed in recombinant E. coli, and is a kind of DNA-dependent 5'→3' RNA polymerase with high specificity for recognizing subsequence of T7 promoter. T7 RNA polymerase can utilize single-stranded or double-stranded DNA of the T7 promoter sequence as a template, and NTPs as substrates to synthesize RNA complementary to the single-stranded or double-stranded DNA template downstream of the promoter.
This kit employs double-antibody sandwich ELISA to quantify of T7 RNA Polymerase. The elisa plate is coated with monoclonal antibody against T7 RNA Polymerase to form the immobilized antibody. The T7 RNA Polymerase standard and the test sample are added to the immobilized antibody microplate. After washing, enzyme-labeled reagents labeled with horseradish peroxidase are added to form the "coated antibody - antigen - enzyme labeled detection antibody" complex. Add the TMB substrate solution after washing to develop color (the TMB substrate solution turns blue under the catalysis of HRP enzyme, and the color finally changes to yellow with an acid. The color intensity is positively correlated with the amount of T7 RNA Polymerase in the sample).
Components
See the component Table above.
Product Manual
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EasyAna T7 RNA Polymerase Quantitative Detection Kit (ELISA) SKU: DD3504EN
Highlights
Features
# Raw materials controllable:
Independent development of antibodies;
Raw materials traceable and controllable;
Good stability, Good quality control.
# Excellent product performance:
High analytical sensitivity-LOD=100 pg/ml, LOQ=500 pg/ml;
Good precison-CV＜15%;
Good accuracy-Standard recovery accuracy meets 80 - 120%;
Good specificity-T7 RNA polymerase-specific recognition with no interference from other IVT enzymes.
# Complete methodological validation:
The kit methodology follows pharmacopoeia and biological sample analysis methods to validate M10 for complete validation.
Can provide methodology verification report to meet the requirements of analytical method verification in the application process.
# Easy to operate:
Using the precoated enzyme label plate (which has been coated with anti-T7 RNA Polymerase monoclonal antibody),
can directly add samples or detection, which is more convenient to use and more stable results.
Storage
BOX 1 is stored at -30 ~ -15°C, away from direct light;
BOX 2 is stored at 2 ~ 8°C, away from direct light.
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