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EasyAna dsRNA (Modified) Quantitative Detection Kit (ELISA) 2.0, SKU: DD3509EN
mRNA QC Reagents: 
 
EasyAna dsRNA (Modified) Quantitative Detection Kit (ELISA) 2.0, SKU: DD3509EN
This kit uses a double-antibody sandwich ELISA method to detect residual double-stranded RNA (dsRNA) in IVT products. 
 
Product Overview
 
The EasyAna dsRNA (modified) Quantitative Detection Kit (ELISA) 2.0 is developed based on a double antibody sandwich ELISA platform and is used to quantitatively detect the amount of dsRNA, a byproduct, in IVT products or synthetic mRNA solutions.
 
Components
See the component Table above.
 
Performance  
 
  • Dilution accuracy
To evaluate the consistency of the test results of samples gradiently diluted to different concentration. The coefficient variation (CV) between the recalculated concentration and the theoretical concentration after different multiples of dilution of 300 ng/ml dsRNA is less than 20%  (Figure 2).
 
 
  • High specificity
We verified that the ssRNA, dsDNA, ssDNA, and the in vitro transcriptional enzymes used during mRNA preparation did not interfere with the detection system within the linear range of 0.046–1.5 ng/ml (Figure 3).
 
  • Inter-lot consistency
High, medium, and low-concentration samples were tested with 3 different lots, 10 times per lot, and the CV of  lot-to-lot is ＜15% (Table 2).
 
Product Manual
 
Manual_DD3509EN_V24.2
 
 
Related products
 
DD3413-C1 - dsRNA-unmodified Standard (High Concentration);
DD3436-02 - dsRNA-Pseudouridine-U (Standard);
DD3437-02 - dsRNA-N1-me-Pseudo-U (Standard)
DD3445-01 - Concentrated wash buffer(20x)
 

EasyAna dsRNA (Modified) Quantitative Detection Kit (ELISA) 2.0, SKU: DD3509EN

SKU: DD3509EN-01
C$2,239.00Price
Quantity

  • Highlights

     

     

     

    Features

     

    • Easy-to-operate:  

    1. Pre-coated ELISA Plates can slash over 20 hours* of coating time and minimize the risk of inaccuracies caused by human handling.

    2. All necessary components are included.

    • Nucleic acid antibody screening techniques:  Antibodies compatible with different dRNA sequences

    • Excellent performance: See Figure 1.

    1. High analytical sensitivity:  LOD < 10 pg/ml; LOQ = 146.87 pg/ml;
    2. High accuracy: Recovery rate between 80% and 120%;
    3. High precision: CV<15%;
    4. High specificity: Specifically identify dsRNA of longer than 40 bp; not interfered by other forms of nucleic acids or IVT enzymes [T7 RNA polymerase, etc.]

     

     

  • Storage

     

     

    Store at 2 ~ 8℃ and protect from direct bright light. 

     

     

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