Dual Luciferase Reporter Assay Kit, SKU: DL101
Dual fluorescence detection, more reliable results
Product Overview
The Dual-Luciferase Reporter Gene Detection Kit is used to detect gene regulation. This kit reflects luciferase expression levels by transfecting cells with a reporter plasmid and measuring the fluorescence intensity of the luciferin substrate. To achieve dual-luciferase reporter gene detection, firefly luciferase is detected using luciferin as a substrate, and Renilla luciferase is detected using coelenterate as a substrate, while simultaneously inhibiting firefly luciferase activity.
The Dual-Luciferase Reporter Gene Detection Kit can sensitively and efficiently detect luciferase expression regulated by gene elements. Typically, transcriptional regulatory elements are cloned upstream of firefly luciferase, or the 3'-UTR regulatory region is cloned downstream of firefly luciferase. Cells are transfected with the appropriate stimulant, and then lysed to measure luciferase activity. The stimulatory-inducing effect of the regulatory element is assessed by luciferase activity. Renilla luciferase is used as an internal control to correct for transfection efficiency, eliminating differences in cell number and transfection efficiency between different wells. Firefly luciferase catalyzes the emission of luciferin at 560 nm, while Renilla luciferase catalyzes the emission of coelenterate at 465 nm.
Components
See above Table.
Product Manual
Dual Luciferase Reporter Assay Kit, SKU: DL101
PRODUCT OVERVIEW
Features
- Strong fluorescence signal: Suitable for detection of weak promoters or low expression regulation.
- Wide detection range: Linear range up to 8 orders of magnitude (R²>0.99).
- High sensitivity: Detection sensitivity as low as 10⁻¹⁸ mol.
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
Dissolved and dispensed Reagent: long-term storage at -85 ~ -65°C, or short-term storage at -30 ~ -15°C for no more than one month.
Stop & Reaction Buffer: long-term storage at 2 ~ 8°C.
