circRNA Pull-Down Kit, SKU: FI8710
Product Description
The independently developed circRNA Pull-Down Kit (patented by the China National Intellectual Property Administration) leverages the high-affinity interaction between the proprietary "F2 tag" and its specific ligand to efficiently capture target circular RNAs (circRNAs) along with their associated proteins or RNAs from cellular environments.
The F2 tag is a short RNA sequence that, when fused with the target circRNA, maintains the native structural integrity and biological functionality of the molecule.
circRNA Pull-Down Kit
The Specialized Circular RNA Solution
Purpose: A highly specialized version of the pull-down assay designed specifically for the unique structural requirements of Circular RNA (circRNA).
Technical Advantage (Junction-Specific Capture): This kit leverages the high-affinity F2 tag system but is optimized for the cellular environment where circRNAs often exist in low abundance compared to their linear counterparts.
Impact: By using the F2-ligand interaction, the kit can specifically isolate the circular isoform and its associated "scaffold" proteins or "microRNA sponges," bridging the gap between transcriptomic discovery and functional proteomics.
Product Manual
circRNA Pull-Down Kit, SKU: FI8710
Highlights
Features
- The F2 tag is small enough to maintain the native folding and secondary structure of the target RNA.
- Start with known circRNA.
- Highly specific binding.
- Low background noisy.
Storage
Store upon arrival at 4 - 8 °C for Box 1 and at -30 - -15°C for Box 2.
