Dye based Real-time Quantitative PCR (qPCR)
ChamQ Blue Universal SYBR qPCR Master Mix, SKU: Q312
Guided by blue, trusted for precision, designed for every platform.
Product Overview
This product is a premium qPCR premix designed for exceptional performance with the SYBR Green I intercalating fluorescence method. Its distinct blue color ensures easy and precise sample handling. At its core is Champagne Taq DNA Polymerase, a cutting-edge antibody-based hot-start DNA polymerase that delivers unparalleled specificity and ultra-high sensitivity. Paired with an advanced, qPCR-optimized buffer and proprietary specificity enhancers, this premix is perfect for achieving highly reliable and sensitive results.The inclusion of a specially formulated ROX Passive Reference Dye ensures seamless compatibility across various qPCR instruments, with no need for ROX concentration adjustments. Just add your primers and templates, and you're ready to experience precise, efficient amplification every time.
Components
See the Table above.
Performance
Broad Quantification Range
Arabidopsis cDNA was subjected to a 5-step, 10-fold serial dilution and amplified using the ChamQ Blue Universal SYBR qPCR Master Mix (Vazyme #Q312) to target the GAPDH gene (instrument: ABI QuantStudio 3). The results demonstrated that Vazyme #Q312 exhibits excellent linearity across a wide range of template concentrations. See the above Figure 1 .
High Amplification Specificity
Using 293 gDNA and HeLa cDNA as templates, three different genes were amplified under identical reaction conditions with Vazyme #Q312 and competitor dye-based qPCR reagents (instrument: ABI QuantStudio 3). The results demonstrated that Vazyme #Q312 exhibited higher specificity compared to similar products across various amplification systems. See the above Figure 2 -Gene 1, Gene 2 and Gene 3.
Strong Program Compatibility
Figure3 A compares the reaction times of Vazyme #Q312 under standard and fast cycling protocols on various instruments.
Figure3 B shows the expression analysis of a target gene using rice leaf cDNA subjected to a 5-step, 10-fold serial dilution. Amplifications were performed with Vazyme #Q312 under both standard and fast cycling conditions (instrument: ABI QuantStudio 3). The results indicate that Vazyme #Q312 is highly compatible with both standard and fast quantification protocols. See the above Figure 3.
Product Manual
ChamQ Blue Universal SYBR qPCR Master Mix, SKU: Q312
Highlights
Features
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Blue Visual Guidance
"Precision you can see, for a more intuitive and reliable qPCR experience." With blue dye providing real-time visual cues, this mastermix effectively reduces pipetting errors, making it especially suitable for high-throughput workflows and beginner users.
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High Sensitivity Detection
"The perfect choice for low-abundance genes." An optimized formulation ensures clear amplification curves and accurate quantification results, even at low template concentrations.
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Versatility and Stability
"One mix for all platforms and sample types." Precisely optimized for broad compatibility, it supports DNA, cDNA, and various sample types. Designed for stable performance and long-term storage peace of mind.
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Storage
Store at -30 ~ -15°C and protect from light. Transport at ≤0°C. After thawing, the Master mix can be stored stably for 6 months at 2 ~ 8°C and protected from light.
▲ If white precipitate is found in the Master Mix after thawing, place it at room temperature for a short while and invert the tube upside down several times to dissolve the precipitate. Please make sure the precipitates have fully dissolved and mix thoroughly before use.
