CellCounting-Lite 3D Luminescent Cell Viability Assay, SKU: DD1102
CellCounting-Lite 3D (CCL3D) is a cell viability assay that detects the amount of ATP released by micro-tissue cell clump, sphenoid or organoids under 3D cell culture conditions.
 
Product Overview
CellCounting-Lite 3D (CCL3D) is a cell viability assay based on a luciferase system. This reagent contains high-purity luciferin, thermostable luciferase, and optimized reaction reagents, resulting in enhanced lysis capabilities and making it more suitable for micro-tissue cell clump samples under 3D cell culture conditions. 
 
Adding this product to cell cultures causes cell clumps to lyse and release ATP, resulting in the reaction shown in Figure 1 and emitting a stable light signal. Within a certain range, the luminescence intensity is directly proportional to the ATP content (i.e., the number of viable cells). Therefore, this product can be used for the quantitative detection of viable cell counts.
 
Detection Workflow
See CellCounting-Lite 3D detection principle and workflow (Figure 1 and 2).
 
Product Manual
Manual_DD1102-V20.1 
 
Citation
95/100 Bioz Rating, 49  Citations by May 14, 2026. See the updated citation link.
Highlight
1. High-fidelity bioassembly of organoids and spheroids using inertial droplet microfluidics for precision oncology and tumor microenvironment modeling.
Yuchen Li, Zemei Cao, ..., Wenbin Du
Microsystems & Nanoengineering, 2026 Apr 24, https://www.nature.com/articles/s41378-026-01244-x 
2. Multi-omics driven immune classification of colorectal cancer: Implications for immunotherapy efficacy prediction and enhancement with WNT signaling inhibition.
Chuling Hu, Du Cai, ..., Feng Gao
Cancer letters, 2026 Mar 11, https://doi.org/10.1016/j.canlet.2026.218425 
3. Intestinal Lachnospiraceae bacterium-derived propionate inhibits the progression of clear cell renal cell carcinoma.
Jun-Yao Xu, Huan Chen, ..., Wei Zhai
Cell Reports Medicine,  2025 Oct 08,  DOI: 10.1016/j.xcrm.2025.102410
4. Erythrabyssin II is identified as a novel late-stage autophagy inhibitor that reverses chemoresistance and promotes apoptosis in ovarian cancer.
Jing Mo, Qiling Cai, ..., Yang Sun
iScience, 2025 Jun 01,  DOI: 10.1016/j.isci.2025.112801
5. Stepwise activation of SARM1 for cell death and axon degeneration revealed by a biosynthetic NMN mimic.
Yinpin Huang, Jun Zhang, ..., Xiaodong Wang
Proceedings of the National Academy of Science,  2025 Feb 18,   DOI: 10.1073/pnas.2424906122
6. Targeting CDK2 Confers Vulnerability to Lenvatinib Via Driving Senescence in Anaplastic Thyroid Cancer.
Ben Ma, Youzhou Sang, ..., Yu Wang
Advanced Science, 2024 Dec 24, DOI: 10.1002/advs.202413514 
7. Clinical functional proteomics of intercellular signalling in pancreatic cancer.
Peiwu Huang, Weina Gao, ..., Ruijun Tian
Nature, 2024 Nov 13, https://www.nature.com/articles/s41586-024-08225-y
8. Neoadjuvant sintilimab plus chemotherapy in EGFR-mutant NSCLC: Phase 2 trial interim results (NEOTIDE/CTONG2104).
Chao Zhang, Yu-Xuan Sun, ..., Wen-Zhao Zhong
Cell Reports Medicine, 2024 Jul 17, DOI: 10.1016/j.xcrm.2024.101615