CellCounting-Lite 3D Luminescent Cell Viability Assay, SKU: DD1102
CellCounting-Lite 3D (CCL3D) is a cell viability assay that detects the amount of ATP released by micro-tissue cell clump, sphenoid or organoids under 3D cell culture conditions.
Product Overview
CellCounting-Lite 3D (CCL3D) is a cell viability assay based on a luciferase system. This reagent contains high-purity luciferin, thermostable luciferase, and optimized reaction reagents, resulting in enhanced lysis capabilities and making it more suitable for micro-tissue cell clump samples under 3D cell culture conditions.
Adding this product to cell cultures causes cell clumps to lyse and release ATP, resulting in the reaction shown in Figure 1 and emitting a stable light signal. Within a certain range, the luminescence intensity is directly proportional to the ATP content (i.e., the number of viable cells). Therefore, this product can be used for the quantitative detection of viable cell counts.
Detection Workflow
See CellCounting-Lite 3D detection principle and workflow (Figure 1 and 2).
Product Manual
Citation
95/100 Bioz Rating, 56 Citations by Nov 01, 2026. See the updated citation link.
Highlights
HIF-1 signaling contributes to lenvatinib resistance in patient-derived HCC organoids.
Yongye Huang, Zhu Ai, Senmiao Huang
Genomics | 2026 Sep 04 | PMID 42697385 | Read Article
UFL1‐Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer Progression
Xiuqing Ma, Rui Wan, ..., Tongzheng Liu
Advanced Science | 2026 Jul 29 | PMID 42523085 | Read Article
High-fidelity bioassembly of organoids and spheroids using inertial droplet microfluidics for precision oncology and tumor microenvironment modeling.
Yuchen Li, Zemei Cao, ..., Wenbin Du
Microsystems & Nanoengineering, 2026 Apr 24, https://www.nature.com/articles/s41378-026-01244-x
Multi-omics driven immune classification of colorectal cancer: Implications for immunotherapy efficacy prediction and enhancement with WNT signaling inhibition.
Chuling Hu, Du Cai, ..., Feng Gao
Cancer letters, 2026 Mar 11, https://doi.org/10.1016/j.canlet.2026.218425
Intestinal Lachnospiraceae bacterium-derived propionate inhibits the progression of clear cell renal cell carcinoma.
Jun-Yao Xu, Huan Chen, ..., Wei Zhai
Cell Reports Medicine, 2025 Oct 08, DOI: 10.1016/j.xcrm.2025.102410
Erythrabyssin II is identified as a novel late-stage autophagy inhibitor that reverses chemoresistance and promotes apoptosis in ovarian cancer.
Jing Mo, Qiling Cai, ..., Yang Sun
iScience, 2025 Jun 01, DOI: 10.1016/j.isci.2025.112801
5. Stepwise activation of SARM1 for cell death and axon degeneration revealed by a biosynthetic NMN mimic.
Yinpin Huang, Jun Zhang, ..., Xiaodong Wang
Proceedings of the National Academy of Science, 2025 Feb 18, DOI: 10.1073/pnas.2424906122
Targeting CDK2 Confers Vulnerability to Lenvatinib Via Driving Senescence in Anaplastic Thyroid Cancer.
Ben Ma, Youzhou Sang, ..., Yu Wang
Advanced Science, 2024 Dec 24, DOI: 10.1002/advs.202413514
Clinical functional proteomics of intercellular signalling in pancreatic cancer.
Peiwu Huang, Weina Gao, ..., Ruijun Tian
Nature, 2024 Nov 13, https://www.nature.com/articles/s41586-024-08225-y
Neoadjuvant sintilimab plus chemotherapy in EGFR-mutant NSCLC: Phase 2 trial interim results (NEOTIDE/CTONG2104).
Chao Zhang, Yu-Xuan Sun, ..., Wen-Zhao Zhong
Cell Reports Medicine, 2024 Jul 17, DOI: 10.1016/j.xcrm.2024.101615
CellCounting-Lite 3D Luminescent Cell Viability Assay, SKU: DD1102
Highlights
Features
Simpler operation: Single component, one-step process.
Easier for daily use: No separate freeze-thaw cycle required.
Stronger lysis capability: More precise quantification.
Storage
Long-term storage at -30 to -15°C. Transport at -20 to 0°C.
