Add＆Read TR-FRET Human IL7 Quantitative Detection Kit, SKU: DD2711
Add&Read Human IL7 is based on TR-FRET technology, also known as "wash-free ELISA". Compared with traditional ELISA, it only requires two steps, saving more than 80% of time and labor.
 
Product Overview
Interleukin-7 (IL-7) is a pleiotropic cytokine primarily produced by bone marrow stromal cells. Its main functions include promoting the proliferation of B cells and T cells and exerting anti-apoptotic effects. IL-7 plays a crucial role in T cell proliferation, survival, and differentiation, and also possesses potential chemotactic activity. Like other cytokines, IL-7 is an immunomodulatory protein, playing a vital role in the normal development of the immune system and the maintenance of normal immune function.

The Add＆Read TR-FRET Human IL7 Quantitative Detection Kit uses a sandwich assay to detect IL-7 levels. This kit contains an IL-7 standard and two monoclonal antibodies that specifically recognize IL-7. One antibody is conjugated to europium (Eu) (donor, Anti-IL-7-Eu), and the other antibody is conjugated to A3 (A3) (receptor, Anti-IL-7-A3). When both antibodies bind to IL-7 simultaneously, Anti-IL-7-Eu and Anti-IL-7-A3 approach each other, resulting in fluorescence resonance energy transfer (FRET) (Figure 1). The fluorescent donor was excited by 320/340 nm light, emitting 620 nm light. This 620 nm light then excited the fluorescent acceptor, causing it to emit 665 nm light. The concentration of IL-7 in the sample was proportional to the FRET signal value (the ratio of the 665 nm and 620 nm light intensities).
 
Product Manual
DD2711 Manual V25.1