Add&Read TR-FRET Human IL2 Quantitative Detection Kit, SKU: DD2705Add&Read Human IL2 is based on TR-FRET technology, also known as "wash-free ELISA". Compared with traditional ELISA, it only requires two steps, saving more than 80% of time and labor.
Product Overview
Interleukin-2 (IL-2) is an important immunomodulatory cytokine, primarily produced by activated T cells. It plays a crucial role in promoting the proliferation of T lymphocytes and natural killer (NK) cells, stimulating B cell differentiation and proliferation, and enhancing antibody production. IL-2 is essential for immune responses, immunomodulation, and antitumor immunity in vivo.
The Add&Read TR-FRET Human IL2 Quantitative Detection Kit uses a sandwich assay to detect IL-2 levels. The kit contains IL-2 standards and two IL-2-specific monoclonal antibodies, one conjugated to Eu (donor, Anti-IL-2-Eu) and the other conjugated to A3 (receptor, Anti-IL-2-A3). When both antibodies bind simultaneously to IL-2, the proximity of Anti-IL-2-Eu and Anti-IL-2-A3 enables fluorescence resonance energy transfer (FRET) (Figure 1). The fluorescent donor is excited using 320/340 nm excitation light, emitting light at a wavelength of 620 nm. The fluorescent acceptor is excited by 620 nm light, emitting light at 665 nm. The concentration of IL2 in the sample was proportional to the FRET signal (the ratio of fluorescence intensity at 665 nm and 620 nm).
Product Manual
Add&Read TR-FRET Human IL2 Quantitative Detection Kit, SKU: DD2705
Highlights
Features
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Easy to operate: Just add sample and incubate at room temperature and read plate.
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Save sample: Only 16μL is needed.
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Fast: Save 80% time and labor compared with traditional ELISA.
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High-throughput: Suitable for automated instruments.
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Storage
The reagent kit should be stored at -30 ~ -15℃ and transported at ≤0℃
