Add&Read Human IgG Kit, SKU: DD2101Add&Read Human IgG Kit: The Add & Read method based on TR-FRET technology is also known as "wash-free ELISA". Compared with traditional ELISA, it only requires two steps, saving more than 80% of time and labor.
Product Overview
This Add&Read Human IgG Assay kit is designed to detect the concentration of human IgG in cell supernatant or purified cells. The kit contains two antibodies that recognize human IgG: one antibody recognizing the Fc region, labeled with the luminescent donor Europium (Anti-hFc-Eu); and another antibody recognizing the Fab region, labeled with the luminescent acceptor A2 (Anti-hFab-A2).
When Anti-hFc-Eu and Anti-hFab-A2 come into close contact (binding occurs), Fluorescence Resonance Energy Transfer (FRET) occurs. (See Figure 1.) The luminescent donor Europium is excited at 320 nm light, emitting light at 620 nm, which in turn excites the luminescent acceptor A2, emitting light at 665 nm. The concentration of the human IgG being measured is directly proportional to the FRET signal value (the ratio of the light intensities at 665 nm and 620 nm).
Product Manual
DD2101-01-V20.1 for DD2101-01 (500 tests, 50 x)
DD2101-02-V20.1 for DD2101-02 (10,000 tests, 50 x)
Add&Read Human IgG Kit, SKU: DD2101
Highlights
Features
- Sandwich assay for monoclonal antibody detection
- Good subtype compatibility, high consistency with ELISA and HPLC results
- High sensitivity: detection range 0.91~2000 ng/ml
Storage
The Human IgG Kit should be stored at temperatures between -30 ~ -15℃, and transported at temperatures ≤0℃.
