Add&Read cAMP Detection Kit, SKU: DD2801
Add&Read cAMP Detection Kit: The Add & Read method based on TR-FRET technology is also known as "wash-free ELISA". Compared with traditional ELISA, it only requires two steps, saving more than 80% of time and labor.
 
Product Overview
The cAMP assay kit is a competitive immunoassay used to detect the accumulation of intracellular cyclic adenosine monophosphate (cAMP). The kit contains an antibody that specifically recognizes cAMP, labeled with a fluorescent donor Eu (Anti-cAMP-Eu), while cAMP is labeled with a fluorescent acceptor A2 (cAMP-A2).
 
In solution, Anti-cAMP-Eu binds to cAMP-A2, resulting in fluorescence resonance energy transfer (FRET). When excited with 320 nm light, the fluorescent donor Eu emits light at 620 nm. This 620 nm light, in turn, excites the fluorescent acceptor A2, causing it to emit light at 665 nm. After cell lysis, intracellularly produced cAMP is released into the lysis sample, competitively binding with anti-cAMP-Eu and disrupting the FRET phenomenon. The FRET signal value (the ratio of light intensity at 665 nm to 620 nm) is inversely proportional to the concentration of cAMP in the standard or sample.
 
Product Manual
Manual_DD2801 V24.1