One Step qRT-PCR Probe Kit
 
AccurSTART II U+ One Step RT-qPCR Super Premix (Fast & Single Tube), SKU: Q631
Designed for Seamless RT-qPCR, Powered by Pre-Assembled Stability.
 
PRODUCT OVERVIEW
AccurSTART II U+ One-Step RT-qPCR Super Premix (Fast and Single Tube) is a fully pre-assembled, probe-based master mix designed for single-tube, one-step RT-qPCR applications. It is optimized for rapid and sensitive quantification of RNA templates (e.g., RNA viruses) in both singleplex and multiplex assays.

This premix utilizes an engineered hot-start enzyme system that combines a modified reverse transcriptase with a novel high-affinity anti-Taq antibody. This ensures precise enzyme activation, minimizes nonspecific amplification, and improves reaction accuracy. The premix also contains temperature-activated reverse transcriptase and an upgraded chemical buffer system, allowing for pre-loading of primers and probes. Once formulated into working solution, the premix can be stored at low temperatures for extended periods and is ready for use without additional tube opening or pipetting steps.

Q631 is compatible with rapid cycling protocols, significantly reducing overall assay time. It also includes a dUTP/UDG contamination control system that operates effectively at room temperature, preventing residual contamination and ensuring reliable results.
 
Performance
 
Fully Preassembled, One-Step Reaction
      See the Figure above.
 
Confident Multiplexing Without Compromising Sensitivity or Workflow
Q631 is engineered for high-sensitivity multiplex detection. It enables accurate amplification of low-copy viral RNA across multiple targets in a single reaction. The formulation includes optimized enzymes and buffer systems that reduce inhibition and improve compatibility across both singleplex and multiplex assays. Validated across commonly used fluorescence channels such as FAM, VIC, and ROX, Q631 maintains strong signal intensity without the typical drop in performance often seen in multiplex reactions. Even when fully pre-mixed with primers and probes, it consistently delivers high-quality results. This ensures convenient deployment without compromising sensitivity or reliability.
 
Signal intensity comparison across three singleplex systems and a multiplex system. Our multiplex formulation (right) achieves consistent fluorescence across multiple channels with performance comparable to or better than individual singleplex setups.
 
High Sensitivity — Maintained in Fast Protocol
Amplification curve comparison between the fast protocol and standard RT-qPCR setup. The rapid protocol achieves comparable sensitivity with significantly shorter run time.
 
Products manual
Manual_Q631 V25.1