High-Throughput Automated DNA and RNA Extraction
VAMNE Magnetic Cell/Tissue Total RNA Kit, SKU: RMA3301-01
One Less Pretreatment Step, One Leap in Quality — RNA Success Is “Mag”nified!
Product Overview
VAMNE Magnetic Cell/Tissue Total RNA Kit (Prepackaged) is designed for the extraction of high-purity RNA from cells and animal tissues, achieving high-throughput processing of samples. The kit is based on superparamagnetic particle purification technology with an optimized reagent system, which can effectively remove various impurities and specifically bind nucleic acids. DNase I can efficiently eliminate DNA. The fully automated workflow is simple, rapid, and safe. The obtained RNA can be directly used for downstream molecular biology applications such as RT-PCR, qRT-PCR, Northern blot, Dot blot, in vitro translation, and Next-generation sequencing.
Components
See the component table above.
Performance
Workflow
See the streamlined workflow above (Figure1).
Excellent Performance
We evaluated the performance of RMA3302 (paired with Vazyme #VNP-32P) and RMA3303 (paired with Vazyme #VNP-96P) against comparable products from Supplier A/B, as well as Vazyme’s own precipitation-based RNA extraction kit (#R411) and column-based RNA extraction kit (#RC112) (Figure 2). Testing was performed across a variety of tissue and cell samples, with RNA products analyzed via electrophoresis and purity assessment.
Results demonstrate that RNA isolated with RMA3302 and RMA3303 exhibits clear, bright bands on the electrophoresis gel. The OD260/280 and OD260/230 ratios are close to standard values, confirming high RNA purity.
According to the quantification and 4200 TapeStation analysis of RNA isolated from PBMC samples with varying input amounts (Figure 3), the results demonstrated that RNA from all tested inputs exhibited consistently high integrity with RIN values above 9 — with RMA3302 and RMA101-C2 delivering particularly stable and high integrity scores up to 9.6 — while RMA3302 also showed excellent purity, as evidenced by its high A260/280 ratios (≥2.00) and A260/230 ratios (peaking at 1.98), and clear, intact bands on TapeStation electrophoresis profiles further confirmed robust RNA quality across all sample input levels.
Product Manual
VAMNE Magnetic Cell/Tissue Total RNA Kit, SKU: RMA3301-01
Highlights
Features
High yield, integrity, and purity.
Fast and high throughput: Only 5 min manual operation, get 96 samples in 45 min.
Visualization: Color indication of optimum buffer mixing.
Broad compatibility: Compatible with various plasmid types and multiple host strains.
Low endotoxin: Ensure downstream experiment success rate >99%.
Storage
BOX 1: Store at 15 ~ 25°C and ship at room temperature.
BOX 2: Store at -30 ~ -15°C and ship at ≤0°C.
Frequently Asked Questions
Frequently Asked Questions
Q1. Are there any incompatible samples?
A1. Special attention should be paid to spleen and pancreas tissue samples. Due to the relatively high content of endogenous RNA in such samples, if the RIN value of the extracted RNA is required to be greater than 7, it is recommended to use Vazyme #R411 reagent for extraction. After adding chloroform , transfer the RNA phase to the extraction plate for purification.
Q2. How much lysis buffer should be added when cells are in a 96-well plate?
A1. First, prepare the pre-processing solution, which consists of 470μl lysis buffer, 20μl proteinase K and 20μl buffer TP. Then take 200μl from the 520μl pre-processing solution to treat the samples
Q3. If higher concentration is needed and the volume of elution buffer is reduced, what is the minimum volume that can be reduced to?
A3. If pre-packaged plate reagents are used, the reagent has been prefilled with approximately 70μl of elution buffer; if reduction is needed, it can be reduced to 40-50μl as the initial elution buffer volume. After extraction, the volume of the final product is approximately 20-35μl.
