CellCounting-Lite 2.0 Luminescent Cell Viability Assay, SKU: DD1101
CellCounting-Lite 2.0 (CCL2) is a cell viability assay that detects the amount of ATP released by living cells through a luminescent signal based on a luciferase system.

Product Overview
CellCounting-Lite 2.0 (CCL2) is a cell viability detection reagent based on the luciferase system. The reagent contains high-purity luciferin and thermostable luciferase. 
 
When this product is added to the cell culture, the cells will lyse and release ATP, and the reaction shown in Figure 1 will occur, emitting a stable "glow" signal. The luminescence intensity is proportional to the amount of ATP, that is, the number of living cells, within a certain range, so this product can be used for quantitative detection of the number of living cells.
 
Detection Workflow
See CellCounting-Lite 2.0 detection principle and workflow (Figure 1 and 2).
 
Product Manual
Manual_DD1101_V20.1
 
Citation
95/100 Bioz Rating, 70  Citations by May 11, 2026.  Please see the updated citations.
Highlight
1. SLC13A2-transported citrate remodels transcriptional regulation through protein acetylation to suppress tumor growth.
Mengyao Qin, Longcheng Shang, ..., Jing Xiong
Science Advances, 2026 Apr 10. DOI: 10.1126/sciadv.aec4368 
2. Timosaponin AIII enhances CAR-T cell potency and prevents relapse through impairing CAR-Tregs.
Mingqi Hou, Wenjun Zhang, ..., Jing Yang
Nature Communications, 2026 Mar 31, https://www.nature.com/articles/s41467-026-70867-5
3. Wogonin derivative V8 enhances bortezomib efficacy in gastric carcinoma by disrupting lysosome-mediated drug resistance.
Si-Chan Li, Shun-Zi Shao, ..., Xiao-Bo Zhang
World Journal of Gastroenterology,  2026 Feb 10. DOI: 10.3748/wjg.v32.i8.113299 
4. The role of histone demethylase PHF2 as a tumour suppressor in hepatocellular carcinoma by regulating SRXN1.
Dexter Kai Hao Thng, Lissa Hooi, ..., Edward Kai-Hua Chow
Oncogenesis, 2026 Jan 19, https://www.nature.com/articles/s41389-026-00597-2
5. PFKM phosphorylates histone H3 and promotes mitotic progression by sensing the levels of citrate.
Pianpian Lin, Yijun Qi, ..., Weiwei Yang
Nature communications, 2025 Jul 22. https://www.nature.com/articles/s41467-025-62111-3
6. Clinical functional proteomics of intercellular signalling in pancreatic cancer.
Peiwu Huang, Weina Gao, ..., Ruijun Tian
Nature, 2024 Nov 13, https://www.nature.com/articles/s41586-024-08225-y
7. Designer cellular spheroids with DNA origami for drug screening.
Jiayi Wei, Yueyang Sun, ..., Hao Pei
Science Advances,  2024 Jul 19, https://www.science.org/doi/10.1126/sciadv.ado9880 
8. Molecular subtypes of neuroendocrine carcinomas: A cross-tissue classification framework based on five transcriptional regulators.
Zhanyu Wang, Chengming Liu, ..., Jie He
Cancer cell, 2024 Jun 11, DOI: 10.1016/j.ccell.2024.05.002